Cerebellar Fastigial Nuclear Inputs and Peripheral Feeding Signals Converge on Neurons in the Dorsomedial Hypothalamic Nucleus
Cerebellar Fastigial Nuclear Inputs and Peripheral Feeding Signals Converge on Neurons in the Dorsomedial Hypothalamic Nucleus
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小脑顶核输入和外周进食信号汇聚在下丘脑背内侧核的神经元上
DOI：
10.1159/000197913
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发表时间：
2009-01-01
期刊：
影响因子：
--
通讯作者：
Wang, Jian-Jun
中科院分区：
文献类型：
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作者：
Li, Bin;Guo, Chun-Li;Wang, Jian-Jun
以往的研究表明，下丘脑背内侧核（DMN）的神经元接收来自胃迷走神经、血糖和瘦素的摄食相关信号。另一方面，小脑通过直接的小脑-下丘脑投射参与调节非躯体内脏活动，包括食物摄入，这是一个有趣的发现。本研究旨在通过大鼠体内的细胞外记录，检测小脑顶核（FN）是否能与进食相关的胃迷走神经、血糖和瘦素信号到达并汇聚到单个DMN神经元上。在记录的200个DMN神经元中，104个（52%）对小脑FN刺激有反应，其中95个（91.3%）也对胃迷走神经刺激有反应，表明小脑FN和胃迷走神经输入在DMN神经元上汇合。此外，当同时刺激小脑FN和胃迷走神经时，观察到反应的总和（n = 10）。在接受小脑FN和胃迷走神经趋同输入的18个DMN神经元中，16个（88.9%）细胞也对葡萄糖和瘦素的全身给药有反应。这些结果表明，小脑fn传入输入与胃迷走神经、血糖和瘦素的摄食信号一起汇聚到单个DMN神经元上，提示DMN中可能发生了与摄食相关的躯体-内脏整合，小脑可能通过小脑FN-DMN投射积极参与摄食调节。
Previous studies have indicated that neurons in the dorsomedial hypothalamic nucleus (DMN) receive feeding-related signals from the gastric vagal nerves, glycemia as well as leptin. On the other hand, it is intriguing that the cerebellum participates in regulating nonsomatic visceral activities including food intake via the direct cerebellohypothalamic projections. The present study was designed to examine, by using extracellular recordings in vivo in rats, whether the cerebellar fastigial nucleus (FN) could reach and converge with the feeding-associated gastric vagal, glycemia and leptin signals onto single DMN neurons. Of the 200 DMN neurons recorded, 104 (52%) responded to the cerebellar FN stimulation, in which 95 (91.3%) were also responsive to the gastric vagal stimulation, suggesting a convergence of cerebellar FN and gastric vagal inputs on the DMN neurons. Moreover, a summation of responses was observed (n = 10) when the cerebellar FN and gastric vagal nerve were simultaneously stimulated. Among the 18 DMN neurons receiving convergent inputs from the cerebellar FN and gastric vagal nerves, 16 (88.9%) cells also responded to the systemic administrations of glucose and leptin. These results demonstrated that the cerebellar FN-afferent inputs, together with the feeding signals from the gastric vagal nerves, blood glucose as well as leptin, converge onto single DMN neurons, suggesting that a somatic-visceral integration related to the feeding may occur in the DMN and the cerebellum may actively participate in the feeding regulation through the cerebellar FN-DMN projections.