Tissue-based map of the human proteome
Tissue-based map of the human proteome
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DOI：
10.1126/science.1260419
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发表时间：
2015-01-23
期刊：
影响因子：
56.9
通讯作者：
Ponten, Fredrik
中科院分区：
文献类型：
--
作者：
Uhlen, Mathias;Fagerberg, Linn;Ponten, Fredrik
解决人体不同组织和器官中蛋白质组变异的分子细节将大大增加我们对人类生物学和疾病的了解。在这里，我们基于综合组学方法绘制了人类组织蛋白质组图，该方法涉及组织和器官水平的定量转录组学，并结合基于组织微阵列的免疫组织化学，以实现蛋白质的空间定位直至单细胞水平。我们基于组织的分析检测到超过90%的推定蛋白质编码基因。我们用这种方法探索了人类分泌组、膜蛋白质组、药物蛋白质组、癌症蛋白质组以及32种不同组织和器官中的代谢功能。所有数据都集成在一个基于Web的交互式数据库中，该数据库允许探索人体所有主要组织和器官中的单个蛋白质，并导航整体表达模式。
Resolving the molecular details of proteome variation in the different tissues and organs of the human body will greatly increase our knowledge of human biology and disease. Here, we present a map of the human tissue proteome based on an integrated omics approach that involves quantitative transcriptomics at the tissue and organ level, combined with tissue microarray-based immunohistochemistry, to achieve spatial localization of proteins down to the single-cell level. Our tissue-based analysis detected more than 90% of the putative protein-coding genes. We used this approach to explore the human secretome, the membrane proteome, the druggable proteome, the cancer proteome, and the metabolic functions in 32 different tissues and organs. All the data are integrated in an interactive Web-based database that allows exploration of individual proteins, as well as navigation of global expression patterns, in all major tissues and organs in the human body.