Mitogenic activity of Actinomyces viscosus. I. Effects on murine B and T lymphocytes, and partial characterization.
Mitogenic activity of Actinomyces viscosus. I. Effects on murine B and T lymphocytes, and partial characterization.
复制标题
粘性放线菌的有丝分裂活性。
作者：
D. Engel;J. Clagett;R. Page;B. Williams
粘放线菌匀浆（AVIS）含有一种物质，这种物质会导致来自常规和无菌小鼠的脾脏细胞经历增加的DNA合成。这种有丝分裂作用主要发生在B细胞上，因为来自裸小鼠的脾脏细胞或来自常规小鼠的T-耗尽脾脏细胞的反应与常规（T + B）脾脏细胞一样强烈。小鼠胸腺细胞对AVIS无丝分裂反应。有丝分裂活性不太可能是由于脂多糖的存在，因为粘单胞菌是革兰氏阳性的，并且不知道是否有脂多糖细胞壁成分。此外，AVIS不会像一些LPS制剂那样被多粘菌素B灭活，C3H/HeJ小鼠脾细胞对AVIS反应强烈，而对LPS反应不强烈。活性是热稳定的，在透析时不会丧失，并且不受溶菌酶的影响。当AVIS被非特异性细菌蛋白酶消化或用超碘酸盐处理时，有丝分裂活性部分丧失。氢氧化钠处理完全消除有丝分裂活性。放线菌病的特点是长期的炎症反应，包括致密的浆细胞浸润。我们认为放线菌形成的B细胞有丝分裂原可能在这些病变的浆细胞成分的激发中起作用。
Actinomyces viscosus homogenate (AVIS) contins substance(s) which cause spleen cells from conventional and germfree mice to undergo increased DNA synthesis. This mitogenic effect is primarily on B cells since spleen cells from nude mice or T-depleted spleen cells from conventional mice respond as strongly as conventional (T + B) spleen cells. Mouse thymocytes do not respond mitogenically to AVIS. It is unlikely that the mitogenic acitivity is due to the presence of LPS, since A. viscosus is Gram-positive and is not known to have an LPS cell wall component. Also, AVIS is not inactivated by polymyxin B, as are some preparations of LPS, and C3H/HeJ mouse splenocytes respond strongly to AVIS but not to LPS. The activity is heat stable, is not lost upon dialysis, and is not affected by lysozyme. Mitogenic activity is partially lost when AVIS is digested with nonspecific bacterial protease or treated with metaperiodate. Sodium hydroxide treatment completely abolishes mitogenic activity. Actinomycotic lesions are characterized by a long-tern inflammatory response involving a dense plasma cell infiltrate. We suggest that B cell mitogens form Actinomyces may play a role in the elicitation of the plasma cell component of these lesions.