GABA modulates cytotoxicity of immunocompetent cells expressing GABAA receptor subunits
GABA modulates cytotoxicity of immunocompetent cells expressing GABAA receptor subunits
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DOI：
10.1016/s0753-3322(98)80019-x
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发表时间：
1998-01-01
影响因子：
7.5
通讯作者：
Chany, C
中科院分区：
文献类型：
--
作者：
Bergeret, M;Khrestchatisky, M;Chany, C
C57黑色小鼠脾T淋巴细胞效应细胞与Balb/c小鼠脾细胞共培养致敏；然后添加 P815 DBA 小鼠肥大细胞瘤靶细胞并测定特异性 T 细胞依赖性细胞毒性。在用γ-氨基丁酸（GABA）处理致敏效应物后，这种细胞毒性增加，但在用GABA处理靶标后，这种细胞毒性降低。这些 GABA 效应似乎是特定的，因为它们被线性而非分支的 GABA 类似物部分模仿。此外，它们很可能是由 GABA(A) 受体介导的，因为 GABA(A) 受体亚基 mRNA 和蛋白质可以在效应细胞或靶免疫特异性细胞中得到证实，这表明在尚未确定的情况下，GABA 可能会影响 T 细胞功能。 (C) 1998 爱思唯尔，巴黎。
C57 black mouse splenic T lymphocytes effector cells were co-cultivated with Balb/c mouse splenic cells for sensitization; P815 DBA mouse mastocytoma target cells were then added and specific T cell-dependent cytotoxicity determined. This cytotoxicity increased after gamma-aminobutyric acid (GABA) treatment of the sensitized effecters, hut decreased after GABA treatment of the targets. These GABA effects seemed to be specific since they were partially mimicked by linear but not ramified GABA analogues. Furthermore, they were likely mediated by GABA(A) receptor since GABA(A) receptor subunit mRNAs and protein could be demonstrated in effector or target immune specific cells, suggesting that under yet to be defined circumstances, GABA may affect T cell functions. (C) 1998 Elsevier, Paris.