Influence of histatin 5 on Candida albicans mitochondrial protein expression assessed by quantitative mass spectrometry.
Influence of histatin 5 on Candida albicans mitochondrial protein expression assessed by quantitative mass spectrometry.
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DOI：
10.1021/pr100861k
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发表时间：
2011-02-04
影响因子：
4.4
通讯作者：
Oppenheim, Frank G.
中科院分区：
文献类型：
--
作者：
Komatsu, Tomoko;Salih, Erdjan;Helmerhorst, Eva J.;Offner, Gwynneth D.;Oppenheim, Frank G.
关键词：
人唾液抗真菌蛋白组胺素5的作用模式的各个方面已部分阐明，但其机制可能涉及一组尚未表征的复杂事件。以前的证据指向组胺诱导的线粒体功能改变。本研究的目的是验证和量化的变化，线粒体蛋白质组的C。用组胺素处理的白色念珠菌5.通过平板接种测定细胞杀伤，并通过采用mTRAQ和ICAT标记和Western印迹的定量蛋白质组学方法测定线粒体样品中的差异蛋白质表达水平。建立了144种不同蛋白质的相对定量比。上调的线粒体蛋白主要参与基因组的维持和基因表达，而构成呼吸酶复合物的蛋白则大多下调。通过Western印迹法，通过与组胺素处理后未改变的细胞色素c水平进行比较，证实了ATP合酶γ链和延伸因子1-α的差异表达。mTRAQ和ICAT蛋白质组学结果表明，组胺素5抗真菌机制的关键步骤涉及C.白色念珠菌，主要由线粒体ATP合成减少引起。
Individual aspects of the mode of action of histatin 5, a human salivary antifungal protein, have been partially elucidated, but the mechanism likely involves a complex set of events which have not been characterized. Previous evidence points toward histatin-induced alterations in mitochondrial function. The purpose of the present study was to verify and quantify changes in the mitochondrial proteome of C. albicans treated with histatin 5. Cell killing was determined by plating and differential protein expression levels in the mitochondrial samples were determined by quantitative proteomics approaches employing mTRAQ and ICAT labeling and Western blotting. Relative quantitation ratios were established for 144 different proteins. Up-regulated mitochondrial proteins were predominantly involved in genome maintenance and gene expression, whereas proteins that constitute the respiratory enzyme complexes were mostly down-regulated. The differential expression of ATP synthase gamma chain and elongation factor 1-alpha were confirmed by Western blotting by comparison to levels of cytochrome c which were unchanged upon histatin treatment. The mTRAQ and ICAT proteomics results suggest that key steps in the histatin 5 antifungal mechanism involve a bioenergetic collapse of C. albicans, caused essentially by a decrease in mitochondrial ATP synthesis.
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影响因子：
11.8
作者：
Edmond, MB;Wallace, SE;Wenzel, RP
通讯作者：
Wenzel, RP
影响因子：
4.1
作者：
Helmerhorst, EJ;VantHof, W;Amerongen, AVN
通讯作者：
Amerongen, AVN
影响因子：
4.8
作者：
Helmerhorst, EJ;van't Hof, W;Oppenheim, FG
通讯作者：
Oppenheim, FG
影响因子：
10.5
作者：
Easlon, Erin;Tsang, Felicia;Lin, Su-Ju
通讯作者：
Lin, Su-Ju
DOI：
10.1073/pnas.141366998
发表时间：
2001-12-04
影响因子：
11.1
作者：
Helmerhorst, EJ;Troxler, RF;Oppenheim, FG
通讯作者：
Oppenheim, FG