Foxd3 mediates zebrafish myf5 expression during early somitogenesis
Foxd3 mediates zebrafish myf5 expression during early somitogenesis
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DOI：
10.1016/j.ydbio.2005.11.032
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发表时间：
2006-02-15
影响因子：
2.7
通讯作者：
Tsai, HJ
中科院分区：
文献类型：
--
作者：
Lee, HC;Huang, HY;Tsai, HJ
Myf 5是一种基本的螺旋-环-螺旋转录因子，控制肌肉的分化，在胚胎早期的体节中表达。然而，与myf 5的顺式元件结合的转录因子知之甚少。在这项研究中，我们使用酵母单杂交试验，发现叉头盒d3（Foxd 3）与-82/-62盒，指导myf 5的体节特异性表达的关键元件特异性相互作用。双荧光素酶测定显示Foxd 3的表达有效地反式激活myf 5启动子。用morpholino oligonucleotide（MO）敲低foxd 3导致myf 5在体节和近轴细胞中显著下调，但在体前中胚层中没有。另一方面，myod的表达在foxd 3变体中保持不变。foxd 3介导myf 5的表达是阶段依赖性的，在7- 18体节阶段维持myf 5在体节和近轴细胞中的表达。此外，在pax 3 morphant中，foxd 3的表达显著下调，myf 5的表达与foxd 3 morphant相似。共注射foxd 3 mRNA和pax 3-MOI大大恢复了myf 5在体节和近轴细胞中的表达，表明pax 3诱导foxd 3表达，foxd 3然后诱导myf 5的表达。这份报告是第一个研究表明，Foxd 3，一个众所周知的调节神经嵴发育，也参与myf 5的调节。(C)2005年爱思唯尔公司All rights reserved.
Myf5, one of the basic helix-loop-helix transcription factors, controls muscle differentiation and is expressed in somites during early embryogenesis. However, the transcription factors bound to the cis-elements of myf5 are poorly understood. In this study, we used the yeast one-hybrid assay and found that Forkhead box d3 (Foxd3) interacted specifically with the -82/-62 cassette, a key element directing somite-specific expression of myf5. The dual-luciferase assay revealed that the expression of Foxd3 potently transactivated the myf5 promoter. Knocking down foxd3 with morpholino oligonucleotide (MO) resulted in a dramatic down-regulation of myf5 in somites and adaxial cells but not in the presomitic mesoderm. On the other hand, myod expression remained unchanged in foxd3 morphants. Foxd3 mediation of myf5 expression is stage-dependent, maintaining myf5 expression in the somites and adaxial cells during the 7- to 18-somite stage. Furthermore, in the pax3 morphant, the expression of foxd3 was down-regulated greatly and the expression of myf5 was similar to that of the foxd3 morphant. Co-injection of foxd3 mRNA and pax3-MOI greatly restored the expression of myf5 in the somites and adaxial cells, suggesting that pax3 induces foxd3 expression, which then induces the expression of myf5. This report is the first study to show that Foxd3, a well-known regulator in neural crest development, is also involved in myf5 regulation. (C) 2005 Elsevier Inc. All rights reserved.