Human dermal fibroblasts present tetanus toxoid antigen to antigen-specific T cell clones.
Human dermal fibroblasts present tetanus toxoid antigen to antigen-specific T cell clones.
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人真皮成纤维细胞将破伤风类毒素抗原呈递给抗原特异性 T 细胞克隆。
DOI：
10.1172/jci111955
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发表时间：
1985
影响因子：
15.9
通讯作者：
R. Geha
中科院分区：
文献类型：
--
作者：
D. Umetsu;J. Pober;H. Jabara;W. Fiers;E. Yunis;S. Burakoff;C. Reiss;R. Geha
用免疫干扰素处理的培养的人真皮成纤维细胞表达HLA-DR抗原。我们在这里报告，DR阳性成纤维细胞目前破伤风类毒素（TT）的自体TT特异性单克隆辅助T细胞大力耗尽单核细胞通过Sephadex G10柱，然后用单克隆抗体（mAb）OKM 1和Leu M1加补体治疗。DR阳性成纤维细胞对TT的反应性T细胞增殖的程度与单核细胞作为抗原呈递细胞的反应性T细胞增殖的程度相似。增殖反应是TT依赖性的，抗原特异性的，依赖于成纤维细胞的DR表达，表现为MHC限制性的，并且被针对HLA-DR的小鼠mAb完全阻断，但不被针对HLA-A、B或DQ的mAb阻断。DR阳性成纤维细胞脉冲TT同样有效的抗原呈递。总之，免疫干扰素刺激的人真皮成纤维细胞可以替代经典的抗原呈递细胞的抗原特异性增殖反应。由于成纤维细胞是体内普遍存在的细胞类型，它们可能在宿主的免疫生物学中发挥重要作用。
Cultured human dermal fibroblasts treated with immune interferon express HLA-DR antigens. We report here that DR-positive fibroblasts present tetanus toxoid (TT) to autologous TT-specific monoclonal helper T cells vigorously depleted of monocytes by passage over Sephadex G10 columns followed by treatment with the monoclonal antibodies (mAb) OKM1 and Leu M1 plus complement. The extent of T cell proliferation in response to TT presented by DR-positive fibroblasts was similar to that elicited using monocytes as antigen-presenting cells. The proliferative response was TT dependent, antigen specific, depended upon DR expression by fibroblasts, appeared MHC restricted, and was completely blocked by mouse mAb to HLA-DR but not by mAb to HLA-A,B, or DQ. DR-positive fibroblasts pulsed with TT were similarly effective in antigen presentation. In summary, immune interferon-stimulated human dermal fibroblasts can substitute for classical antigen-presenting cells in antigen-specific proliferative responses. Since fibroblasts are a ubiquitous cell type in the body, they may play a significant role in the immunobiology of the host.