Identification of Tn 10 insertions in the rfaG, rfaP, and galU genes involved in lipopolysaccharide core biosynthesis that affect Escherichia coli adhesion
Identification of Tn 10 insertions in the rfaG, rfaP, and galU genes involved in lipopolysaccharide core biosynthesis that affect Escherichia coli adhesion
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DOI：
10.1007/s002030050732
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发表时间：
1999-07-01
影响因子：
2.8
通讯作者：
Oudega, B
中科院分区：
文献类型：
--
作者：
Genevaux, P;Bauda, P;Oudega, B
以大肠杆菌为模型，研究了其在非生物表面的初始粘附和早期生物膜的形成。选择大肠杆菌K-12 W3110的Tn 10插入突变体以改变粘附于聚苯乙烯表面的能力。七个插入突变体，表现出减少粘附窝藏插入的基因参与脂多糖（LPS）的核心生物合成。两个插入位于rfaG基因中，两个在rfaP基因中，三个在galU基因中。这些粘附突变体被发现表现出深粗糙的表型，并在不同的水平上减少，在1型菌毛的生产和运动。这些突变体表现出的粘附力的损失与受影响的1型菌毛生产和/或功能障碍的运动。除了影响LPS对1型菌毛和鞭毛生物合成的突变的多效性作用外，没有观察到LPS本身参与粘附到聚苯乙烯表面的证据。
Escherichia coli was used as a model to study initial adhesion and early biofilm development to abiotic surface. Tn10 insertion mutants of Escherichia coli K-12 W3110 were selected for altered abilities to adhere to a polystyrene surface. Seven insertion mutants that showed a decrease in adhesion harbored insertions in genes involved in lipopolysaccharide (LPS) core biosynthesis. Two insertions were located in the rfaG gene, two in the rfaP gene, and three in the galU gene. These adhesion mutants were found to exhibit a deep-rough phenotype and to be reduced, at different levels, in type 1 fimbriae production and motility. The loss of adhesion exhibited by these mutants was associated with either the affected type 1 fimbriae production and/or the dysfunctional motility. Apart from the pleiotropic effect of the mutations affecting LPS on type 1 fimbriae and flagella biosynthesis, no evidence for an involvement of the LPS itself in adhesion to polystyrene surface could be observed.