Humoral immunity to commensal oral bacteria in human infants: evidence that Streptococcus mitis biovar 1 colonization induces strain-specific salivary immunoglobulin A antibodies.
Humoral immunity to commensal oral bacteria in human infants: evidence that Streptococcus mitis biovar 1 colonization induces strain-specific salivary immunoglobulin A antibodies.
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人类婴儿对共生口腔细菌的体液免疫：轻链球菌生物变种 1 定植诱导菌株特异性唾液免疫球蛋白 A 抗体的证据。
DOI：
10.1038/ismej.2008.26
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发表时间：
2008
期刊：
影响因子：
--
通讯作者：
Cole,MichaelF
中科院分区：
文献类型：
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作者：
Wirth,KatherineA;Bowden,GeorgeH;Kirchherr,JenniferL;Richmond,DorothyA;Sheridan,MichaelJ;Cole,MichaelF
为了确定唾液SIgA抗体与口腔共生细菌之间的关系，我们检测了四名婴儿唾液中SIgA抗体的反应性，这些婴儿的唾液中都有各自的混合型链球菌(S.mitisbv1)克隆(核型)。免疫印迹分析被用来检测这些抗体与产后一年内从婴儿口腔中分离出的持久性核糖体类型的反应性。结果表明，定植后与大多数克隆的SIgA抗体反应模式的复杂性增加，但大多数额外的条带是其他克隆所共有的，表明它们代表共同的抗原。然而，在75%的选定持久克隆中发现了独特的条带。我们假设，如果菌株特异性SIgA抗体是针对特定克隆的定植而诱导的，并有助于将其从口腔中消除，那么独特条带的出现将立即在菌株消失之前出现。在研究中确定的所有独特带子中，有73%符合这一标准。由于口腔是一个开放的、动态的环境，多种因素被认为在粘膜表面的免疫反应中发挥作用，因此可能无法确定SIgA抗体与共生菌之间的关系。然而，我们的数据提供了证据，即SIgA抗体与其自身定植株的独特抗原反应，在婴儿中产生，并可能指出该抗体在调节定植中的作用。Mitisbv 1.
To define the relationship between salivary SIgA antibodies and commensal oral bacteria, we examined the reactivity of SIgA antibodies from the saliva of four infants with their own colonizingStreptococcus mitisbiovar 1 (S. mitisbv 1) clones (ribotypes). Immunoblot analysis was used to examine reactivity of these antibodies with persistent ribotypes isolated from the mouths of the infants over the first yearpostpartum. Results showed that the pattern of SIgA antibody reactivity with the majority of clones increased in complexity after colonization but that most additional bands were common to other clones, indicating that they represented shared antigens. However, unique bands were identified in 75% of the selected persistent clones. We hypothesized that if strain-specific SIgA antibody was induced in response to colonization of a particular clone and contributed to its elimination from the mouth, then the appearance of unique bands would immediately precede the disappearance of the strain. Seventy-three percent of all unique bands identified in the study fulfilled this criterion. Because the mouth is an open, dynamic environment and multiple factors are believed to play a role in the immune response at mucosal surfaces, it may not be possible to conclusively define the relationship between SIgA antibody and commensal bacteria. However, our data provide evidence that SIgA antibody, reactive with unique antigens of their own colonizing strains, is produced in infants and may point to a role of this antibody in regulating colonization byS. mitisbv 1.