Platelet activation in mice and human Helicobacter pylori infection
Platelet activation in mice and human Helicobacter pylori infection
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DOI：
10.1172/jci119650
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发表时间：
1997-09-01
影响因子：
15.9
通讯作者：
Pique, JM
中科院分区：
文献类型：
--
作者：
Elizalde, I;Gomez, J;Pique, JM
幽门螺杆菌（HP）提取物已被证明可诱导肠系膜小静脉中的白细胞粘附，但HP感染对胃微血管的影响尚不清楚。用活体视频显微镜观察了分别接种生理盐水或新鲜分离HP的小鼠胃微循环中炎症细胞的相互作用。小鼠门静脉血中血小板聚集物的检测和定量使用双放射标记单抗技术检测内皮细胞p -选择素的表达，通过测量血小板聚集比和血小板p -选择素的表达来研究hp感染患者和对照组的血小板活化和聚集。HP感染小鼠胃小静脉中滚动白细胞的通量、血小板和白细胞-血小板聚集物的出现明显增加，HP诱导的滚动白细胞和血小板聚集物的形成被抗l -或P-的单克隆抗体所消除，但对e-选择素没有作用，内皮细胞中P-选择素的表达没有改变，但血小板中P-选择素的表达增强。在HP感染患者中也检测到循环血小板聚集和活化血小板，这些发现表明血小板活化和聚集有助于HP感染相关的微血管功能障碍和炎症细胞募集。
Extracts of Helicobacter pylori (HP) have been shown to induce leukocyte adhesion in mesenteric venules, but the effects of HP infection on gastric microvessels are unknown. inflammatory cell interactions in the gastric microcirculation were studied by intravital videomicroscopy in mice inoculated with either saline or fresh isolates of HP. Platelet aggregates were detected and quantified in murine portal blood? while endothelial P-selectin expression was determined using the dual radiolabeled mAb technique, Platelet activation and aggregation were studied in HP-infected patients and controls by measuring the platelet-aggregate ratio and platelet P-selectin expression.HP infection induced a marked increase in the flux of rolling leukocytes and the appearance of platelet and leukocyte-platelet aggregates in murine gastric venules, The HP-induced rolling and platelet aggregate formation was abrogated by mAbs against L-or P-, but not E-selectin, Endothelial cell expression of P-selectin was not altered, but platelet P-selectin expression was enhanced in HP-infected mice, Circulating platelet aggregates and activated platelets were also detected in HP-infected patients, These findings indicate that platelet activation and aggregation contribute to the microvascular dysfunction and inflammatory cell recruitment associated with HP infections.