Bacterial Diversity in Amblyomma americanum (Acari: Ixodidae) With a Focus on Members of the Genus Rickettsia
Bacterial Diversity in Amblyomma americanum (Acari: Ixodidae) With a Focus on Members of the Genus Rickettsia
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DOI：
10.1603/me09197
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发表时间：
2010-03-01
影响因子：
2.1
通讯作者：
Little, S. E.
中科院分区：
文献类型：
--
作者：
Heise, Stephanie R.;Elshahed, M. S.;Little, S. E.
据报道，孤星蜱 Amblyomma americanum（螨虫：蜱科）在美国东部地区的人类和动物中常见，并且与许多新出现的疾病的传播有关。为了更好地定义孤星蜱内的微生物群落，使用全细菌引物进行基于 16S rRNA 基因的分析，然后对单个克隆 (n = 449) 进行测序，以识别菌落饲养和野生美洲蜱中最常见的细菌操作分类单位 (OTU)。群体饲养的蜱虫主要含有与柯克斯体属成员 (89%; 81/91)、蜱虫常见内共生体和短杆菌属成员 (11%; 10/91) 相关的序列。同样，对未喂食的野生孤星蜱前克隆的分析表明，所有鉴定的 OTU 中有 96.7% (89/92) 属于类柯克斯体内共生体，而在喂食后野生孤星蜱中鉴定的 OTU 中只有 5.1-11.7% (5/98-9/77)。相比之下，被鉴定为立克次氏体的 OTU 的比例。野生捕获的蜱虫在进食前增加了 2.2% (2/92)，进食后高达 46.8% (36/77)，并且所有立克次体属都增加了。恢复的序列与从斑疹热立克次体，特别是 R. amblyommii 和 R. Massiliae 中描述的序列最相似。通过聚合酶链反应、克隆和 17 kDa 和 gltA 基因测序对立克次体蜱群落进行的其他表征证实了这些初步发现，并表明新型立克次体属属。这些蜱虫中可能存在。这些数据提供了对野生孤星蜱的总体以及立克次体群落的深入了解，并可能最终有助于鉴定由美洲蜱传播的新型病原体。
The lone star tick, Amblyomma americanum (Acari: Ixodidae), is commonly reported front people and animals throughout the eastern U.S. and is associated with transmission of a number of emerging diseases. To better define the microbial communities within lone star ticks, 16S rRNA gene based analysis using bacteria-wide primers, followed by sequencing of individual clones (n = 449) was Used to identify the most common bacterial operational taxonomic units (OTUs) present within colony-reared and wild A. americanum. The colony-reared ticks contained primarily sequence affiliated with members of the genus Coxiella (89%; 81/91), common endosymbionts of ticks, and Brevibacterium (11%; 10/91). Similarly, analysis of clones front unfed wild lone star ticks revealed that 96.7% (89/92) of all the OTUs identified were affiliated with Coxiella-like endosymbionts, as compared with only 5.1-11.7% (5/98-9/77) of those identified from wild lone star ticks after feeding. In contrast, the proportion of OTUs identified as Rickettsia sp. in wild-caught ticks increased front 2.2% (2/92) before feeding to its high as 46.8% (36/77) after feeding, and all Rickettsia spp. sequences recovered were most similar to those described from the spotted fever group Rickettsia, specifically R. amblyommii and R. massiliae. Additional characterization of the Rickettsiales tick community by polymerase chain reaction, cloning, and sequencing of 17 kDa and gltA genes confirmed these initial findings and suggested that novel Rickettsia spp. are likely present in these ticks. These data provide insight into the overall, as well as the rickettsial community of wild lone star ticks and may ultimately aid in identification of novel pathogens transmitted by A. americanum.