Respiratory syncytial virus A genotype classification based on systematic intergenotypic and intragenotypic sequence analysis
Respiratory syncytial virus A genotype classification based on systematic intergenotypic and intragenotypic sequence analysis
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DOI：
10.1038/s41598-019-56552-2
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发表时间：
2019-12-27
影响因子：
4.6
通讯作者：
Noyola, Daniel E.
中科院分区：
文献类型：
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作者：
Carlos Munoz-Escalante, Juan;Comas-Garcia, Andreu;Noyola, Daniel E.
呼吸道合胞病毒（RSV）是下呼吸道感染的主要原因，分为两大类（A和B），其中有多种基因型。自该病毒鉴定以来，已记录到RSV基因型时空分布的连续变化。然而，目前还没有确定的基因型定义标准，这影响了对病毒进化、免疫和疫苗开发的理解。我们对4,353个RSV-A G基因胞外区序列进行了系统发育分析，并使用1,103个全基因组序列分析了RSV-A基因的总数。进行基因型内和基因型间p-距离分析和与特定基因型相关的分子标记的鉴定。我们的研究结果表明，以前报道的基因型可以分为九个不同的基因型：GA 1-GA 7，SAA 1，和NA 1。我们建议使用所有基因型的广泛参考序列对G基因胞外域进行分析，以进行准确的基因型鉴定。
Respiratory syncytial virus (RSV), a leading cause of lower respiratory tract infections, is classified in two major groups (A and B) with multiple genotypes within them. Continuous changes in spatiotemporal distribution of RSV genotypes have been recorded since the identification of this virus. However, there are no established criteria for genotype definition, which affects the understanding of viral evolution, immunity, and development of vaccines. We conducted a phylogenetic analysis of 4,353 RSV-A G gene ectodomain sequences, and used 1,103 complete genome sequences to analyze the totallity of RSV-A genes. Intra- and intergenotype p-distance analysis and identification of molecular markers associated to specific genotypes were performed. Our results indicate that previously reported genotypes can be classified into nine distinct genotypes: GA1-GA7, SAA1, and NA1. We propose the analysis of the G gene ectodomain with a wide set of reference sequences of all genotypes for an accurate genotype identification.