19F-NMR study of the effect of lead on intracellular free calcium in human platelets.
19F-NMR study of the effect of lead on intracellular free calcium in human platelets.
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19F-NMR 研究铅对人血小板细胞内游离钙的影响。
DOI：
10.1016/s0167-4889(97)90010-0
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Gupta,RK
中科院分区：
文献类型：
--
作者：
Dowd,TL;Gupta,RK
铅已被证明会影响钙的体内平衡。然而，没有证据表明低浓度铅（≈1μM）对任何人体组织细胞内游离Ca2+浓度的影响。我们利用19f - nmr和氟化细胞内Ca2+指示剂研究了铅对人血小板细胞内游离Ca2+浓度的影响。我们报告细胞内游离Ca2+的基本值为172±8 nM。1、5、10和25 μM Pb2+处理导致细胞内游离Ca2+平均分别增加39%、91%、135%和172%。细胞内游离Ca2+的增加百分比与Pb2+浓度的对数呈线性正相关。使用原子吸收光谱法，总钙的显著增加约为。25 μM Pb2+处理后的血小板中蛋白含量为10 nmol/mg。这表明外部Ca2+的流入有助于观察到的游离Ca2+的增加。该结果为先前报道的铅对血小板功能的影响提供了解释，并提出了低水平铅诱发高血压的机制。
Lead has been shown to affect calcium homeostasis. However, there is no prior evidence to indicate an effect of low concentrationsof lead in the environment (≈1μM) on the intracellular free Ca2+concentration in any human tissue. We have investigated the effect of lead on the intracellular free Ca2+concentration of human blood platelets using19F-NMR and a fluorinated intracellular Ca2+indicator. We report a basal intracellular free Ca2+value of 172 ± 8 nM. Treatment with 1, 5, 10 and 25 μM Pb2+resulted in average increases in intracellular free Ca2+of 39%, 91%, 135% and 172%, respectively. The percent increase in intracellular free Ca2+was linearly and positively correlated with the log of Pb2+concentration. Using atomic absorption spectroscopy, a significant increase in total calcium of approx. 10 nmol/mg protein was found in 25 μM Pb2+treated platelets. This indicates that influx of external Ca2+contributes to the observed increase in free Ca2+. The results provide an explanation for the previously reported effects of lead on platelet function, and suggest a mechanism for low level lead-induced hypertension.