Identification of a tripartite import signal in the Ewing Sarcoma protein (EWS).
Identification of a tripartite import signal in the Ewing Sarcoma protein (EWS).
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尤文肉瘤蛋白 (EWS) 中三方输入信号的鉴定。
DOI：
10.1016/j.bbrc.2009.10.120
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发表时间：
2009
影响因子：
3.1
通讯作者：
Young,PhilipJ
中科院分区：
文献类型：
--
作者：
Shaw,DebraJ;Morse,Robert;Todd,AdrianG;Eggleton,Paul;Lorson,ChristianL;Young,PhilipJ
尤因肉瘤（EWS）蛋白是一种普遍表达的RNA加工因子，主要定位于细胞核。然而，EWS进入细胞核的机制尚不清楚，不同的报道确定了EWS蛋白内三个独立的输入信号。在这里，我们利用一组截断的EWS蛋白来澄清报道的核定位信号。我们描述了三个c端结构域，它们对EWS核的高效定位很重要：(1)第三个rgg基序；(2)最后10个氨基酸（称为PY-import motif）；(3)锌指图案。虽然这三个领域都涉及核进口，但它们并不能独立地推动gfp部分的有效进口。然而，它们共同形成一个复杂的三方信号，有效地驱动gfp进入细胞核。本研究有助于阐明EWS的输入信号，同时确定RGG-和锌指基序的参与具有广泛的意义。
The Ewing Sarcoma (EWS) protein is a ubiquitously expressed RNA processing factor that localises predominantly to the nucleus. However, the mechanism through which EWS enters the nucleus remains unclear, with differing reports identifying three separate import signals within the EWS protein. Here we have utilized a panel of truncated EWS proteins to clarify the reported nuclear localisation signals. We describe three C-terminal domains that are important for efficient EWS nuclear localization: (1) the third RGG-motif; (2) the last 10 amino acids (known as the PY-import motif); and (3) the zinc-finger motif. Although these three domains are involved in nuclear import, they are not independently capable of driving the efficient import of a GFP-moiety. However, collectively they form a complex tripartite signal that efficiently drives GFP-import into the nucleus. This study helps clarify the EWS import signal, and the identification of the involvement of both the RGG- and zinc-finger motifs has wide reaching implications.