Effect of long-term ovariectomy and estrogen replacement on the expression of estrogen receptor gene in female rats
Effect of long-term ovariectomy and estrogen replacement on the expression of estrogen receptor gene in female rats
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DOI：
10.1530/eje.0.1420307
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发表时间：
2000-03-01
影响因子：
5.8
通讯作者：
Abdel-Rahman, AA
中科院分区：
文献类型：
--
作者：
Mohamed, MK;Abdel-Rahman, AA
目的：雌激素具有广泛的作用，涉及多种靶组织。我们研究了长期卵巢切除术（OVX）和卵巢切除联合17 β-雌二醇治疗（OVX 2）对雌性大鼠靶组织雌激素受体（ER）基因表达水平的影响。设计：本研究使用了三组Sprague-Dawley雌性大鼠：假手术（SO）、OVX和OVXE 2。方法：在开始17 β-雌二醇治疗前2周进行SO和OVX。所有组均从雌二醇治疗开始维持流质饮食12周。从大鼠组织中提取总RNA，采用相对定量逆转录PCR技术比较ER α亚型三组中每个靶组织的ER α mRNA水平。长期OVX后，子宫、肾脏和大脑皮质的ER α表达水平显著增加，而肝脏、小脑、脑干、心脏和胸腹主动脉的SO水平比较。另一方面，12周的治疗OVX大鼠与17 β-雌二醇恢复以前上调的ER α mRNA接近SO水平，除了班轮，其中17 β-雌二醇治疗导致ER α mRNA水平显着增加相比，在SO rates.Conclusions：我们得出结论，ER的调节其配体是组织特异性的。
Objective: Estrogen exerts a wide variety of actions involving many target tissues. We studied the effects of long-term ovariectomy (OVX) and OVX with 17 beta-estradiol treatment (OVXE2) on the level of estrogen receptor (ER) gene expression in target tissues of female rats.Design: Three groups of Sprague-Dawley female rats were utilized in this study: sham operated (SO), OVX and OVXE2. Methods: SO and OVX were performed 2 weeks before starting the 17 beta-estradiol treatment. All groups were maintained on liquid diet for 12 weeks from the time of estradiol treatment. Total RNA was prepared from the tissues of the rats and relative quantitative reverse transcription PCR was utilized to compare the ER alpha-subtype (ER alpha) mRNA level in the three groups for each target tissue.Results: Following long-term OVX, the levels of ER alpha expression showed a significant increase in the uterus, kidney and cerebral cortex and no significant change in the liver, cerebellum, brainstem, heart and thoracic and abdominal aorta compared with their SO levels. On the other hand, a 12-week treatment of OVX rats with 17 beta-estradiol restored the previously upregulated ER alpha mRNA to near SO levels except for the liner where the 17 beta-estradiol treatment resulted in a significant increase in the ER alpha mRNA level compared with that in SO rats.Conclusions: We conclude that the regulation of ERs by its ligand is tissue specific.