Green fluorescent protein facilitates rapid in vivo detection of genetically transformed plant cells
Green fluorescent protein facilitates rapid in vivo detection of genetically transformed plant cells
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DOI：
10.1007/s002990050647
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发表时间：
1999-05-01
影响因子：
6.2
通讯作者：
Grof, CPL
中科院分区：
文献类型：
--
作者：
Elliott, AR;Campbell, JA;Grof, CPL
植物转化事件的早期检测对于快速建立和优化植物转化方案是必要的。我们已经评估了修改后的版本的绿色荧光蛋白（GFP）从Aequorea维多利亚作为植物转化的早期记者使用解剖荧光显微镜与适当的过滤器。GFP表达细胞从四个不同的植物物种（甘蔗，玉米，莴苣，烟草）很容易区分，无论是农杆菌介导的或粒子轰击介导的转化。表达gfp的甘蔗细胞的鉴定允许消除高比例的非表达外植体，并且还使得能够视觉选择分裂的转基因细胞，这是转基因生物体产生的早期步骤。通过在体外可视化表达gfp的组织的能力，使转基因细胞簇的回收简化。
Early detection of plant transformation events is necessary for the rapid establishment and optimization of plant transformation protocols. We have assessed modified versions of the green fluorescent protein (GFP) from Aequorea victoria as early reporters of plant transformation using a dissecting fluorescence microscope with appropriate filters. Gfp-expressing cells from four different plant species (sugarcane, maize, lettuce, and tobacco) were readily distinguished, following either Agrobacterium-mediated or particle bombardment-mediated transformation. The identification of gfp-expressing sugarcane cells allowed for the elimination of a high proportion of non-expressing explants and also enabled visual selection of dividing transgenic cells, an early step in the generation of transgenic organisms. The recovery of transgenic cell clusters was streamlined by the ability to visualize gfp-expressing tissues in vitro.