ISOLATIONS OF SIMBU-GROUP VIRUSES IN IBADAN, NIGERIA 1964-69, INCLUDING NEW TYPES SANGO, SHAMONDA, SABO AND SHUNI
ISOLATIONS OF SIMBU-GROUP VIRUSES IN IBADAN, NIGERIA 1964-69, INCLUDING NEW TYPES SANGO, SHAMONDA, SABO AND SHUNI
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DOI：
10.1080/00034983.1972.11686835
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发表时间：
1972-01-01
影响因子：
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通讯作者：
LEE, VH
中科院分区：
文献类型：
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作者：
CAUSEY, OR;CAUSEY, CE;LEE, VH
材料和方法从屠宰场的动物中收集血样并通过别处描述的方法处理（肯普等人，1971年）。根据常规程序，通过脑内接种幼小鼠来尝试分离病毒（Causey等人，1961年）。从East Mrican病毒研究所获得多组超免疫小鼠腹水或血清，其中一种含有Simbu（SAAR 53）病毒抗体，用于通过补体结合（CF）试验对分离株进行临时鉴定。从康涅狄格州纽黑文耶鲁大学虫媒病毒研究中心（YARU）获得的特定Simbu（SAAR 53）小鼠腹水也用于组鉴定。在Ibadan实验室中，使用每周三次腹膜内注射病毒，然后单次注射肉瘤180/TG细胞，制备每种原型或拓扑型病毒的同种超免疫小鼠腹水。通过中和（N）试验进行类型鉴定。通过Reed和Muench（1938）的方法计算感染性的50%致死剂量（LD 60）终点，滴度和中和指数以dex表示（Halfenstein，1960）。
MATERIALS AND METHODSBlood samples from animals at the abattoirs were collected and processed by methods described elsewhere (Kemp et al., 1971). Attempts to isolate virus were made by intracerebral inoculation of infant mice according to routine procedures (Causey et al., 1961). Multigroup hyperimmune mouse ascitic fluids or sera, one of which contained antibodies to Simbu (SAAR 53) virus, were obtained from the East Mrican Virus Research Institute for provisional identification of isolates by complement-fixation (CF) testing. A specific Simbu (SAAR 53) mouse ascitic fluid, obtained from the Yale Arbovirus Research Unit (YARU), New Haven, Connecticut, also was used for group identification. Homologous hyperimmune mouse ascitic fluid was prepared for each prototype or topotype virus in the lbadan laboratory using three weekly intraperitoneal injections of virus followed by a single injection of sarcoma 180/TG cells. Type identification was made by neutralization (N) testing. The SO% lethal dose (LD 60) end point of infectivity was calculated by the method of Reed and Muench (1938), and titres and neutralization indices are expressed in dex (Haldane, 1960).