Osteoclasts in bone regeneration under type 2 diabetes mellitus
Osteoclasts in bone regeneration under type 2 diabetes mellitus
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DOI：
10.1016/j.actbio.2018.11.052
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发表时间：
2019-01-15
影响因子：
9.7
通讯作者：
Liu, Xiaohua
中科院分区：
文献类型：
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作者：
Hu, Zhiai;Ma, Chi;Liu, Xiaohua
糖尿病（DM）影响着全世界数亿人，骨愈合受损是糖尿病相关的重要并发症。了解糖尿病如何影响破骨细胞的活动及其潜在机制对于开发有效的方法来加速糖尿病患者的骨愈合至关重要。然而，到目前为止，DM对破骨细胞的影响仍然是模糊和有争议的。在本研究中，我们建立了一个接近模拟人类T2DM发病机制的2型糖尿病（T2DM）牙槽骨缺损模型，探讨糖尿病破骨细胞在骨再生过程中的活性。我们发现，高葡萄糖浓度降低了T2DM大鼠破骨细胞的形成，并且破骨细胞的分化和功能受到抑制。在高葡萄糖浓度下，破骨细胞对基质的降解明显减少。体内实验进一步表明，T2DM抑制破骨细胞生成和破骨细胞活性，延缓了T2DM大鼠牙槽骨再生过程中基质的降解。我们的工作阐明了T2DM对破骨细胞的影响，并为设计针对T2DM骨再生的破骨细胞的新型支架材料提供了有价值的见解。骨愈合受损是糖尿病（DM）相关并发症之一。了解糖尿病如何影响破骨细胞活性和支架基质降解对于开发加速糖尿病患者骨愈合的有效方法至关重要。然而，目前，DM对骨缺损区破骨细胞活性和基质降解的影响仍然存在争议和模糊。我们建立了2型糖尿病（T2DM）牙槽骨缺损模型，结果显示T2DM可抑制破骨细胞生成和破骨细胞活性，延缓支架基质降解。我们的工作阐明了T2DM对破骨细胞和基质降解的影响，并为设计针对T2DM骨再生的破骨细胞的新型支架材料提供了见解。(C) 2018材料学报Elsevier Ltd.出版。版权所有。
Diabetes mellitus (DM) affects hundreds of million people worldwide and the impaired bone healing is an important DM-related complication. Understanding how DM affects the activities of osteoclasts and the underlying mechanisms is crucial to the development of effective approaches for accelerating bone healing in DM condition. To date, however, the influence of DM on osteoclasts remains obscure and controversial. In this study, we established a type 2 DM (T2DM) alveolar bone defect model, which closely simulates the pathogenesis of human T2DM, to explore the diabetic osteoclast activity during bone regeneration. We found that a high glucose concentration diminished the formation of osteoclasts, and the differentiation and function of osteoclasts from T2DM rats were suppressed. The degradation of matrix by osteoclasts was significantly reduced at a high glucose concentration. In vivo experiments further indicated that T2DM inhibited osteoclastogenesis and osteoclast activity, and delayed the degradation of matrix during the alveolar bone regeneration in T2DM rats. Our work clarifies the influence of T2DM on osteoclasts, and provides valuable insights for the design of novel scaffolding materials that target on osteoclasts for T2DM bone regeneration.Statement of SignificanceImpaired bone healing is one of the diabetes mellitus (DM)-related complications. Understanding how DM affects osteoclast activity and scaffolding matrix degradation is pivotal to the development of effective approaches for accelerating bone healing in DM condition. Currently, the influences of DM on osteoclast activity and matrix degradation in bone defect areas, however, remain controversial and obscure. Herein, we established a type 2 DM (T2DM) alveolar bone defect model and our results show that T2DM inhibited osteoclastogenesis and osteoclast activity, and delayed the degradation of scaffolding matrix. Our work clarifies the influence of T2DM on osteoclasts and matrix degradation, and provides insights for the design of novel scaffolding materials that target on osteoclasts for T2DM bone regeneration. (C) 2018 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.