Calcein as a Fluorescent Marker of Otoliths of Larval and Juvenile Fish
Calcein as a Fluorescent Marker of Otoliths of Larval and Juvenile Fish
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DOI：
10.1577/1548-8659(1987)116
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发表时间：
1987-07
影响因子：
1.4
通讯作者：
Charles A. Wilson;D. W. Beckman;J. Dean
中科院分区：
文献类型：
--
作者：
Charles A. Wilson;D. W. Beckman;J. Dean
摘要钙黄绿素是一种能与钙等碱土金属结合的荧光化合物。结合后，紫外光下荧光增加。因此，钙黄绿素被评价为潜在的有用的创建一个荧光标记的幼鱼和幼鱼的耳石。在125 mg/L的海水溶液中浸泡2 h后，3种河口鱼类仔、稚鱼的矢状部都产生了这种标记。该标记在紫外荧光显微镜下很容易区分。该技术为年龄和生长研究中使用四环素提供了一种替代方法。
Abstract Calcein is a fluorescent compound that can bind with alkaline earth metals, such as calcium. Upon binding, an increase in fluorescence under ultraviolet light results. Therefore, calcein was evaluated as potentially useful for creating a fluorescent mark in the otoliths of larval and juvenile fish. Such a mark was produced in the sagittae of larvae and juveniles of three species of estuarine fishes after a 2-h immersion in a solution of 125 mg/L in seawater. The mark was easily distinguished under ultraviolet epifluorescent microscopy. This technique provides an alternative to the use of tetracycline in age and growth studies.