Transfer of a parthenogenesis-inducing Wolbachia endosymbiont derived from Trichogramma dendrolimi into Trichogramma evanescens.
Transfer of a parthenogenesis-inducing Wolbachia endosymbiont derived from Trichogramma dendrolimi into Trichogramma evanescens.
复制标题
将源自松毛虫赤眼蜂的单性生殖诱导沃尔巴克氏体内共生体转移到伊氏赤眼蜂中。
DOI：
10.1016/j.jip.2012.09.006
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发表时间：
2012
影响因子：
3.4
通讯作者：
渡部真也
中科院分区：
文献类型：
--
作者：
Watanabe M;Tagami Y;Miura K;Kageyama D;Stouthamer R;渡部真也
沃尔巴克氏体是母系传播的内共生体，被认为在无脊椎动物宿主之间多次水平移动。然而，目前还不清楚沃尔巴克氏体在不同宿主之间水平传播的容易程度，以及水平传播的沃尔巴克氏体在新宿主中建立的频率。我们转移孤雌生殖诱导沃尔巴克氏体内共生体来自寄生蜂赤眼蜂松毛虫赤眼蜂evanescens。具体地说，在蚊子细胞系中培养沃尔巴克氏体，并将沃尔巴克氏体感染的细胞显微注射到未感染的T。evanescens。在276只接种Wolbachia感染细胞的蛹中，出现了65只成虫（G 0）。诊断PCR显示，25 37 G 0女性（68%）沃尔巴克氏体阳性。在从G 0雌性建立的同雌系中，感染株系的比例在G1中为80%（20/25），在G2中为100%（18/18）。在同雌株系中，感染稳定地维持了10代以上。这些结果表明沃尔巴克氏体侵入受体昆虫的生殖系。定量PCR检测结果表明，受体宿主体内的沃尔巴克氏体滴度明显低于天然宿主。受体宿主中孤雌生殖个体的缺乏或非常低的数量（如果有的话）可能是由低沃尔巴克氏体滴度引起的。受体中的沃尔巴克氏体滴度在G11中低于G5，表明密度下降。与以前的报告一起，我们的研究可能意味着赤眼蜂物种中的沃尔巴克氏体高度适应其宿主，这阻碍了沃尔巴克氏体表型在非本地宿主物种中的稳健表达。
Wolbachia, which are maternally transmitted endosymbionts, are considered to have moved horizontally between invertebrate hosts multiple times. However, it is not well understood how easily Wolbachia are transmitted horizontally between different hosts and how frequently horizontally-transmitted Wolbachia become established in their new hosts. We transferred a parthenogenesis-inducing Wolbachia endosymbiont derived from the parasitic wasp Trichogramma dendrolimi to Trichogramma evanescens. Specifically, Wolbachia was cultivated in a mosquito cell line and the Wolbachia-infected cells were microinjected into uninfected T. evanescens. Among 276 pupae inoculated with Wolbachia-infected cells, 65 adults emerged (G0). Diagnostic PCR demonstrated that 25 of 37 G0 females (68%) were Wolbachia-positive. Among isofemale lines established from G0 females, the proportions of infected lines were 80% (20 of 25) in G1 and 100% (18 of 18) in G2. In an isofemale line, infection was stably maintained for more than 10 generations. These results indicate invasion of Wolbachia into the germline of the recipient insect. Quantitative PCR demonstrated that the Wolbachia titer in the recipient host was significantly lower than that in the native host. The absence or very low number, if any, of parthenogenetically-reproducing individuals in the recipient host may be caused by the low Wolbachia titer. The Wolbachia titer in the recipients was lower in G11 than in G5, suggesting a decline in the density. Together with a previous report, our study may imply that Wolbachia in Trichogramma species are highly adapted to their hosts, which hinders robust expression of the Wolbachia phenotype in non-native host species.