Cyclophosphamide decreases the number, percentage and the function of CD25+ CD4+ regulatory T cells, which suppress induction of contact hypersensitivity
Cyclophosphamide decreases the number, percentage and the function of CD25+ CD4+ regulatory T cells, which suppress induction of contact hypersensitivity
复制标题
DOI：
10.1016/j.jdermsci.2005.02.002
复制
发表时间：
2005-08-01
影响因子：
4.6
通讯作者：
Ikezawa, Z
中科院分区：
文献类型：
--
作者：
Ikezawa, Y;Nakazawa, M;Ikezawa, Z
背景：众所周知，在致敏前使用环磷酰胺（Cy）治疗会增强而不是抑制接触性超敏反应（CH）。事实上，cy处理小鼠与未处理小鼠相比，对2,4,6-三硝基-1-氯苯（TNCB）的CH反应显著（p < 0.05）增加。目的：探讨Cy对CD25(+) CD4（+）调节性T细胞的免疫增强作用，探讨Cy对CD25(+) CD4(+) T细胞的影响。方法：采用流式细胞仪和CD25(+) CD4(+) T细胞增殖抑制实验检测cy处理后的CD25(+) CD4(+) T细胞。结果：Cy治疗可显著降低小鼠脾脏和淋巴结中CD25(+) CD4(+) T细胞的数量和百分比。此外，与未处理小鼠相比，3天后从cy处理小鼠身上提取的CD25(+) CD4(+) T细胞对CD25(+) CD4(+) T细胞增殖的抑制活性较低。此外，未处理小鼠的CD25(+) CD4(+) T细胞的转移导致Cy处理增强的CH反应显著减少（p < 0.05）。结论：Cy作用下CH对TNCB反应的增强与CD25(+) CD4（+）调节性T细胞数量、百分比和功能的降低有关。(c) 2005年日本皮肤病研究学会。爱思唯尔爱尔兰有限公司出版。版权所有。
Background: It is well known that cyclophosphamide (Cy) treatment before sensitization paradoxically enhances rather than suppresses contact hypersensitivity (CH) reactions. In fact, Cy-treated mice developed a significant (p < 0.05) increase of the CH reactions to 2,4,6-trinitro-1-chrolobenzene (TNCB) in comparison with untreated mice.Objective: In order to examine whether the target cells of Cy in the immunoaugmentative effect are CD25(+) CD4(+) regulatory T cells or not, we investigated effect of Cy treatment on CD25(+) CD4(+) T cells.Method: We examined Cy-treated CD25(+) CD4(+) T cells by flow cytometer and by inhibition assay on proliferation of CD25(+) CD4(+) T cells.Results: Cy treatment remarkably reduced the number and percentage of CD25(+) CD4(+) T cells in the spleen and lymph nodes 3 and 5 days later. Moreover, CD25(+) CD4(+) T cells taken from the Cy-treated mice 3 days later showed the lower suppressive activity on proliferation of CD25(+) CD4(+) T cells, as compared to that from the untreated mice. Furthermore, transfer of CD25(+) CD4(+) T cells from untreated mice resulted in a significant decrease (p < 0.05) of the CH reactions enhanced by Cy treatment.Conclusion: These results indicate that enhancement of the CH reactions to TNCB by Cy treatment is attributed to the decrease in the number, percentage and the function of CD25(+) CD4(+) regulatory T cells. (c) 2005 Japanese Society for Investigative Dermatology. Published by Elsevier Ireland Ltd. All rights reserved.