Genomic and proteomic analysis of the effects of cannabinoids on normal human astrocytes
Genomic and proteomic analysis of the effects of cannabinoids on normal human astrocytes
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DOI：
10.1016/j.brainres.2007.10.062
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发表时间：
2008-01-29
期刊：
影响因子：
2.9
通讯作者：
Schwartz, S. A.
中科院分区：
文献类型：
--
作者：
Bindukumar, B.;Mahajan, S. D.;Schwartz, S. A.
Delta-9-四氢大麻酚 (Delta(9)-THC) 是大麻的主要精神活性成分，已知会失调各种免疫反应。大麻素（CB）-1和-2受体主要表达于中枢神经系统（CNS）和免疫系统的细胞上。中枢神经系统是大麻素的主要靶标，已知星形胶质细胞在各种免疫反应中发挥作用。因此，我们进行了这项研究，利用基因组和蛋白质组分析确定大麻素对正常人星形胶质细胞（NHA）的整体分子影响。 NHA 用 Delta(9)-THC 处理，并使用基因微阵列和二维 (2D) 差异凝胶电泳 (DIGE) 结合质谱 (MS) 进行分析，以分别阐明其基因组和蛋白质组谱。我们的结果表明，与未经处理的对照 NHA 相比，用 Delta(9)-THC 处理后，NHA 中 20 多种翻译蛋白基因产物的表达出现差异性失调。 (c) 2007 Elsevier B.V. 保留所有权利。
Delta-9-tetrahydrocannabinol (Delta(9)-THC), the main psychoactive component of marijuana, is known to dysregulate various immune responses. Cannabinoid (CB)-1 and -2 receptors are expressed mainly on cells of the central nervous system (CNS) and the immune system. The CNS is the primary target of cannabinoids and astrocytes are known to play a role in various immune responses. Thus we undertook this investigation to determine the global molecular effects of cannabinoids on normal human astrocytes (NHA) using genomic and proteomic analyses. NHA were treated with Delta(9)-THC and assayed using gene microarrays and two-dimensional (2D) difference gel electrophoresis (DIGE) coupled with mass spectrometry (MS) to elucidate their genomic and proteomic profiles respectively. Our results show that the expression of more than 20 translated protein gene products from NHA was differentially dysregulated by treatment with Delta(9)-THC compared to untreated, control NHA. (c) 2007 Elsevier B.V. All rights reserved.