Goniometric measurements of thick tissue using Monte Carlo simulations to obtain the single scattering anisotropy coefficient
Goniometric measurements of thick tissue using Monte Carlo simulations to obtain the single scattering anisotropy coefficient
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DOI：
10.1364/boe.3.002707
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发表时间：
2012-11-01
影响因子：
3.4
通讯作者：
Campagnola, Paul J.
中科院分区：
文献类型：
--
作者：
Hall, Gunnsteinn;Jacques, Steven L.;Campagnola, Paul J.
组织的散射各向异性g可以是组织结构的有力指标，并且可以通过测角法和Henyey-Greenstein相函数拟合来最直接地测量。我们提出了一种方法的基础上的散射系数的独立衰减测量沿着与蒙特卡罗模拟来考虑多重散射，允许准确确定测量的g内的准弹道制度的厚度的组织。模拟结合实验的几何形状和散装光学性质表明，发生在提取g值的显着错误，即使是组织的厚度小于一个散射长度没有建模校正。通过测定小鼠肌肉组织中的g值进行了实验验证，结果表明所获得的值与厚度无关。此外，我们提出了一个简单的反卷积为基础的方法，并表明，它提供了很好的估计高各向异性值（0.95以上）时，再加上一个独立的衰减测量。(C)2012美国光学学会
The scattering anisotropy, g, of tissue can be a powerful metric of tissue structure, and is most directly measured via goniometry and fitting to the Henyey-Greenstein phase function. We present a method based on an independent attenuation measurement of the scattering coefficient along with Monte Carlo simulations to account for multiple scattering, allowing the accurate determination of measurement of g for tissues of thickness within the quasi-ballistic regime. Simulations incorporating the experimental geometry and bulk optical properties show that significant errors occur in extraction of g values, even for tissues of thickness less than one scattering length without modeling corrections. Experimental validation is provided by determination of g in mouse muscle tissues and it is shown that the obtained values are independent of thickness. In addition we present a simple deconvolution-based method and show that it provides excellent estimates for high anisotropy values (above 0.95) when coupled with an independent attenuation measurement. (C) 2012 Optical Society of America