Ultrastructural quantification of cell death after injurious compression of bovine calf articular cartilage
Ultrastructural quantification of cell death after injurious compression of bovine calf articular cartilage
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DOI：
10.1016/j.joca.2003.11.004
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发表时间：
2004-03-01
影响因子：
7
通讯作者：
Hunziker, EB
中科院分区：
文献类型：
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作者：
Patwari, P;Gaschen, V;Hunziker, EB
目的：已经表明，软骨细胞凋亡死亡可能在骨关节炎软骨破坏的发病机制中发挥作用，但体内和体外研究的结果一直相互矛盾。为了进一步研究创伤性关节损伤体外模型中的细胞死亡，我们通过电子显微镜（EM）对损伤性压迫后的细胞形态进行了定量分析。为了比较，TUNEL法也进行了。设计：关节软骨外植体磁盘收获从新生小牛股髌沟。对盘进行损伤性压缩（50%应变，应变速率为100%/s），孵育3天，然后固定用于定量形态学分析。结果：通过TUNEL，细胞凋亡率从未加载对照组的7+/-2%增加到损伤后的33+/-6%（P=0.01; N=8只动物）。通过EM，细胞凋亡率从未负载对照的5+/-1%增加到损伤软骨的62+/-10%（P= 0.02，N=5只动物）。EM分析还确定，在受伤的磁盘中的死细胞，97%凋亡的morphology.Conclusions：这些结果证实了一个显着增加的细胞死亡后，损伤性压缩，并表明，这里观察到的大多数细胞死亡是由凋亡过程。(C)2003年国际骨关节炎研究学会。由爱思唯尔有限公司出版。保留所有权利。
Objective: It has been suggested that chondrocyte death by apoptosis may play a role in the pathogenesis of cartilage destruction in osteoarthritis, but the results of in-vivo and in-vitro investigations have been conflicting. To investigate further the cell death in our in-vitro model for traumatic joint injury, we performed a quantitative analysis by electron microscopy (EM) of cell morphology after injurious compression. For comparison, the TUNEL assay was also performed.Design: Articular cartilage explant disks were harvested from newborn calf femoropatellar groove. The disks were subjected to injurious compression (50% strain at a strain rate of 100%/s), incubated for 3 days, and then fixed for quantitative morphological analysis.Results: By TUNEL, the cell apoptosis rate increased from 7+/-2% in unloaded controls to 33+/-6% after injury (P=0.01; N=8 animals). By EM, the apoptosis rate increased from 5+/-1% in unloaded controls to 62+/-10% in injured cartilage (P=0,02, N=5 animals). Analysis by EM also identified that of the dead cells in injured disks, 97% were apoptotic by morphology.Conclusions: These results confirm a significant increase in cell death after injurious compression and suggest that most cell death observed here was by an apoptotic process. (C) 2003 OsteoArthritis Research Society International. Published by Elsevier Ltd. All rights reserved.