Detection of human enterovirus and human parechovirus (HPeV) genotypes from clinical stool samples: polymerase chain reaction and direct molecular typing, culture characteristics, and serotyping
Detection of human enterovirus and human parechovirus (HPeV) genotypes from clinical stool samples: polymerase chain reaction and direct molecular typing, culture characteristics, and serotyping
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DOI：
10.1016/j.diagmicrobio.2010.05.016
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发表时间：
2010-10-01
影响因子：
2.9
通讯作者：
Wolthers, Katja
中科院分区：
文献类型：
--
作者：
Benschop, Kimberley;Minnaar, Rene;Wolthers, Katja
分子（聚合酶链反应[PCR]）方法越来越多地用于检测和分型人类肠道病毒（HEV）和双埃柯病毒（HPeV）。在这里，我们评估了他们的价值相比，病毒培养和血清分型的检测和分型的HEV和HPeV的粪便样本从住院患者。实时荧光定量PCR检测1174例患者中221例（18.8%）HEV/HPeV阳性。通过细胞培养，可从107份HEV/HPeV PCR阳性样品中分离出病毒。培养效率与Ct值，（基因）类型和所用的细胞系相关。在HEV/HPeV PCR阳性标本中，47%的标本可通过VP 1基因分型进行基因分型，25%的标本可通过血清分型进行基因分型。结论：粪便中HEV/HPeV的PCR检测比病毒培养更敏感，尤其是对柯萨奇病毒A和HPeV。然而，这里使用的基因分型方法只能识别47%的HEV/HPeV毒株。需要进一步优化和验证直接基因分型，并需要确定粪便中HEV/HPeV检测的临床相关性。(C)2010年爱思唯尔公司All rights reserved.
Molecular (polymerase chain reaction [PCR]) methods are increasingly used to detect and type human enteroviruses (HEVs) and parechoviruses (HPeV). Here, we assessed their value in comparison to virus culture and serotyping for detection and typing of HEV and HPeV in stool samples from hospitalized patients. By use of real-time PCR, 221/1174 patients (18.8%) were found positive for HEV/HPeV. By cell culture, a virus could be isolated from 107 of the HEV/HPeV PCR-positive samples. Culture efficiency was correlated to the Ct value, (geno)type, and cell lines used. Of the HEV/HPeV PCR-positive samples, 47% could be genotyped by VP1 genotyping and 25% by serotyping. In conclusion, PCR detection of HEV/HPeV from stool is more sensitive than virus culture, particularly for coxsackieviruses A and HPeVs. However, the genotyping method used here could identify only 47% of the HEV/HPeV strains. Further optimization and validation of direct genotyping are needed, and clinical relevance of HEV/HPeV detection in stool needs to be determined. (C) 2010 Elsevier Inc. All rights reserved.