Pulmonary and serum isotypic antibody responses of mice to live and inactivated influenza virus.
Pulmonary and serum isotypic antibody responses of mice to live and inactivated influenza virus.
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小鼠对活流感病毒和灭活流感病毒的肺和血清同种型抗体反应。
DOI：
10.1164/arrd.1986.134.1.6
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Six,HR
中科院分区：
文献类型：
--
作者：
Balkovic,ES;Six,HR
研究了小鼠呼吸道感染和肌肉注射流感病毒后血清和肺灌洗液中的一、二次免疫球蛋白类特异性抗体反应。感染和接种灭活病毒疫苗可诱导血清和肺灌洗液(PLF)中的IgM和Ig G抗体应答。两种免疫方法均未诱导出可检测到的血清IgA抗体，只有感染才能在PLF中产生IgA抗体。PLF中的抗体主要来源于血清，但在病毒感染后可检测到局部合成的抗体。免疫感染小鼠可提高血清和PLF中的IgM和Ig G抗体浓度，但对IgA抗体浓度无明显影响。非致死性感染疫苗的小鼠在血清和PLF中产生二次IgM和IgG抗体反应，在PLF中产生IgA抗体反应。同样，在PLF中检测到的抗体大多来自血清，但感染后局部合成了低浓度的IgA和IgG。虽然灭活疫苗免疫的小鼠缺乏合成IgA抗体的能力，但当它们受到致命流感病毒的攻击时，它们可以免受严重的肺部疾病的侵袭。这些数据支持血清免疫球蛋白抗体足以预防严重肺部疾病的概念。
Primary and secondary immunoglobulin class-specific antibody responses in serum and pulmonary lavage fluids of mice were studied after respiratory infection and intramuscular vaccination with influenza virus. Infection and vaccination with inactivated virus vaccine induced primary IgM and IgG antibody responses in the serum and pulmonary lavage fluids (PLF). Neither immunizing method induced detectable serum IgA antibodies, and only infection generated IgA antibodies in PLF. The major portion of antibodies in PLF was derived from serum, but local synthesis of IgG and IgA antibodies was detected after virus infection. Vaccination of infection-primed mice boosted the IgM and IgG antibody concentrations in serum and PLF but had no effect on IgA antibody concentrations. Nonlethal infection of vaccine-primed mice generated secondary IgM and IgG antibody responses in serum and PLF and an IgA antibody response in PLF. Again, most of the antibody detected in PLF was derived from serum, but low concentrations of IgA and IgG were synthesized locally after infection. Although mice immunized with inactivated vaccine lacked the capacity to synthesize IgA antibody, they were protected from severe pulmonary disease when challenged with lethal influenza virus. These data support the concept that serum IgG antibodies are sufficient for prevention of severe pulmonary disease.