Participation of CD34-enriched mouse adipose cells in hair morphogenesis
Participation of CD34-enriched mouse adipose cells in hair morphogenesis
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富含CD34的小鼠脂肪细胞参与毛发形态发生
DOI：
10.3892/mmr.2013.1307
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发表时间：
2013-04-01
影响因子：
3.4
通讯作者：
Liu, Wei
中科院分区：
文献类型：
--
作者：
He, Jing;Duan, Huichuan;Liu, Wei
脂肪源性间质血管组分（SVF）细胞本质上是异质的，含有许多不同的细胞类型。最近的研究表明，CD 34可能是脂肪间充质干细胞（ADMSCs）的特异性标志物。以其参与毛发形态发生为模型，研究成体干细胞的多向分化潜能。此外，脂肪组织或成脂谱系细胞似乎与毛囊周期有关。本研究的目的是测试从脂肪组织中富集的CD 34（+）细胞在毛发形态发生中的潜力。为了研究这一点，将未分选的SVF、从绿色荧光蛋白（GFP）转基因小鼠的SVF分选的CD 34（+）和CD 34（-）细胞与胎鼠角质形成细胞和妊娠第17.5天（E17.5）的真皮成纤维细胞混合，然后皮下注射到裸鼠中。结果显示，与其他两组相比，CD 34（+）细胞组的重建皮肤组织块更大，毛囊更发达。组织学和免疫荧光染色分析表明，只有CD 34（+）细胞可能参与毛发形态发生的整合到真皮鞘结构。然而，未观察到其他皮肤附件受累。此外，在CD 34（+）和SVF细胞中也观察到向内皮细胞的分化和参与血管形成，但在CD 34（-）细胞中未观察到。正如预期，在所有组中均观察到参与脂肪形成。我们的研究结果表明，CD 34（+）细胞可能代表ADMSCs，具有更强的多向分化潜能，在重建皮肤发育。
Adipose-derived stromal vascular fraction (SVF) cells are heterogeneous in nature, containing a number of different cell types. Recent studies indicate that CD34 may be a specific marker for adipose-derived mesenchymal stem cells (ADMSCs). Using their participation in hair morphogenesis as a model, the multi-differentiation potential of adult stem cells was investigated. In addition, adipose tissue or adipogenic lineage cells appear to be associated with the hair follicle cycle. The purpose of this study was to test the potential of CD34(+) cells enriched from fat tissue in hair morphogenesis. To investigate this, unsorted SVF, CD34(+) and CD34(-) cells sorted from the SVF of green fluorescence protein (GFP) transgenic mice were mixed with fetal mouse keratinocytes and dermal fibroblasts of gestational day 17.5 (E17.5) and then subcutaneously injected into nude mice. The results showed that in the reconstituted skin tissue, larger tissue blocks with more developed hair follicles were observed in the CD34(+) cell group compared with the other two groups. Histological and immunofluorescent staining analyses revealed that only CD34(+) cells may participate in hair morphogenesis by their integration into dermal sheath structures. However, no involvement in other skin appendages was observed. In addition, differentiation into endothelial cells and participation in blood vessel formation were also observed in both CD34(+) and SVF cells, but not in CD34(-) cells. As expected, participation in adipogenesis was observed in all groups. Our results suggest that CD34(+) cells may represent the ADMSCs which possess stronger multiple differentiation potential during reconstituted skin development.