Analysis of microRNA Expression Detected by Microarray of the Cerebral Cortex After Hypoxic-Ischemic Brain Injury
Analysis of microRNA Expression Detected by Microarray of the Cerebral Cortex After Hypoxic-Ischemic Brain Injury
复制标题
缺氧缺血性脑损伤后大脑皮层微阵列微RNA表达分析
DOI：
10.1097/scs.0b013e3182a243f3
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发表时间：
2013-11-01
影响因子：
0.9
通讯作者：
Yang, Lijun
中科院分区：
文献类型：
--
作者：
Cui, Hong;Yang, Lijun
摘要小分子非编码微RNA（miRNAs）是细胞分化和肿瘤发生等生物学过程的关键调控因子。在缺氧缺血性脑损伤的致病和病理方面，它们也是转录后基因沉默的重要介质。miRNA微阵列被认为是一种高通量、高精度的miRNA表达谱分析工具，为研究miRNA的生物学功能提供了极大的便利。为探讨新生大鼠缺氧缺血性脑损伤（hypoxic-ischemic brain injury，HIBI）后大脑皮质中miRNA表达的变化及其在HIBI发病机制中的可能作用。采用大规模miRNA芯片技术和生物信息学分析方法，比较HIBI大鼠与对照组之间差异表达的miRNA。通过定量实时聚合酶链反应测定3种miRNA（mir-429、mir-200 b和mir-182）的表达。miRNA表达谱分析结果显示，与正常对照组相比，有5种miRNA表达上调2倍以上，29种miRNA表达下调2倍以上。实时荧光定量聚合酶链反应检测3种miRNAs的结果与miRNA芯片检测结果一致。缺氧缺血性脑损伤大鼠脑组织中miRNA表达有明显变化，提示miRNA可能在HIBI的发病机制中发挥重要作用。
Abstract Small and noncoding microRNAs (miRNAs) are known as key regulators of biological processes such as cell differentiation and tumor generation. They are also the important mediators of posttranscriptional gene silencing in both pathogenic and pathologic aspects of hypoxic-ischemic brain injury. miRNA microarray has been considered to be a high-throughput and precise analysis tool for detecting miRNA expression profiling, and it does greatly facilitate the research of the biological function of miRNAs. To investigate the changes of miRNA expression in cortex of neonatal rats with hypoxic-ischemic brain injury (HIBI) and the possible roles of miRNA in the pathogenesis of HIBI, we constructed the model of rat with HIBI and the cortex tissues were obtained 14 days after the HIBI operation. The large-scale miRNA microarrays and bioinformatics analysis were used to determine the differentially expressed miRNAs of HIBI rats compared with controls. Expression of 3 miRNAs (mir-429, mir-200b, and mir-182) was determined by quantitative real-time polymerase chain reaction. The results of miRNA expression profiles indicated that 5 miRNAs were up-regulated more than twice and 29 miRNAs were down-regulated more than twice compared with the normal control group. The results of the 3 miRNAs detected by quantitative real-time polymerase chain reaction were consistent with those detected by miRNA microarray. Hypoxic-ischemic brain injury rats have significant changes in miRNA expression, which demonstrated that miRNAs may play important roles in the pathogenesis of HIBI.