Investigation on cellular interactions of astrocytes with zinc oxide nanoparticles using rat C6 cell lines.
Investigation on cellular interactions of astrocytes with zinc oxide nanoparticles using rat C6 cell lines.
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DOI：
10.1016/j.colsurfb.2015.05.041
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发表时间：
2015-09
期刊：
影响因子：
--
通讯作者：
S. Sruthi;P. Mohanan
中科院分区：
文献类型：
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作者：
S. Sruthi;P. Mohanan
氧化锌纳米粒子（ZnO NPs）广泛应用于化妆品行业，并且在电气和化学工业中也有重要应用。有充分证据表明，吸入的氧化锌纳米粒子可以通过嗅觉神经元通路到达大脑，并可以干扰大脑锌稳态。大多数研究集中在 ZnO NPs 对神经元细胞和小胶质细胞的毒性上。关于 ZnO 纳米颗粒与星形胶质细胞（参与大脑稳态的主要细胞）的生物相互作用的研究还很少。因此，本研究重点关注 ZnO NPs 与大鼠 C6 胶质细胞的相互作用。这项研究的结果表明，纳米颗粒被星形胶质细胞吸收，并诱导时间和剂量依赖性毒理学反应，纳米颗粒吸收研究和细胞活力测定表明了这一点。 DCFH-DA（2',7'-二氯荧光素二乙酸酯）测定结果还表明，ZnO NPs 在 3 小时和 6 小时时会在细胞中诱导强烈的氧化应激。然而，24 小时时，纳米颗粒处理组中检测到的活性氧 (ROS) 与对照组相同。通过核浓缩研究、使用吖啶橙/溴化乙锭的活死实验和细胞凋亡检测试剂盒揭示了ZnO NPs诱导的细胞死亡模式是细胞凋亡。这项研究探讨了 ZnO 纳米颗粒与星形胶质细胞的相互作用，得出的结论是，颗粒的持续存在可能会继续对星形胶质细胞产生破坏性影响。因此，应该更彻底地评估暴露时间和系统清除颗粒的时间，以防止这些颗粒对健康造成危害。
Zinc oxide nanoparticles (ZnO NPs) are widely used in cosmetic industries and have also found important applications in electrical and chemical industries. It is well documented that inhaled ZnO NPs can reach the brain through the olfactory neuronal pathway and can interfere with the brain zinc homeostasis. Most of the studies focus on the toxicity of ZnO NPs on neuronal cells and microglia. Not much work is available on the bio interaction of ZnO NPs with astrocytes, the major cells involved in brain homeostasis. Therefore, this study focuses on the interaction of ZnO NPs with rat C6 glial cells. The results of this study reveal that the nanoparticles are taken up by the astrocytes and induce a time and dose dependent toxicological response which is indicated by the nanoparticle uptake studies and cell viability assays. Also the results of DCFH-DA (2′,7′-dichlorofluorescein diacetate) assay show that the ZnO NPs induce strong oxidative stress in cells at 3 and 6 h. However, at 24 h the reactive oxygen species (ROS) detected in the nanoparticles treated groups were same as that of control. The mode of cell death induced by ZnO NPs was apoptosis as revealed by the nuclear condensation studies, live dead assay using acridine orange/ethidium bromide and apoptosis detection kit. This study, which explores the interaction of ZnO NPs with astrocytes, concludes that the persistence of particle can continue to have a damaging effect on the astrocytes. And hence the time of exposure and particle clearance by the system should be evaluated more thoroughly to prevent the health hazards due to these particles.