Overview of DNA microarrays: types, applications, and their future.
Overview of DNA microarrays: types, applications, and their future.
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DOI：
10.1002/0471142727.mb2201s101
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发表时间：
2013-01
影响因子：
--
通讯作者：
Bumgarner, Roger
中科院分区：
文献类型：
--
作者：
Bumgarner, Roger
本章提供了DNA微阵列的概述。微阵列是一种技术，其中1000个核酸结合到表面上，并用于通过杂交和随后检测杂交事件来测量混合物中核酸序列的相对浓度。我们首先介绍了微阵列的历史和导致其发展的先行技术。然后，我们讨论了微阵列的制造方法和最常见的生物学应用。本章最后简要讨论了微阵列的局限性，并讨论了微阵列如何被DNA测序技术迅速取代。
This chapter provides an overview of DNA microarrays. Microarrays are a technology in which 1000’s of nucleic acids are bound to a surface and are used to measure the relative concentration of nucleic acid sequences in a mixture via hybridization and subsequent detection of the hybridization events. We first cover the history of microarrays and the antecedent technologies that led to their development. We then discuss the methods of manufacture of microarrays and the most common biological applications. The chapter ends with a brief discussion of the limitations of microarrays and discusses how microarrays are being rapidly replaced by DNA sequencing technologies.