A MULTIPLICITY OF CCAAT BOX-BINDING PROTEINS
A MULTIPLICITY OF CCAAT BOX-BINDING PROTEINS
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DOI：
10.1016/0092-8674(87)90513-7
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发表时间：
1987-09-11
期刊：
影响因子：
64.5
通讯作者：
MATHIS, D
中科院分区：
文献类型：
--
作者：
DORN, A;BOLLEKENS, J;MATHIS, D
核因子-Y是一种识别Y盒的序列特异性DNA结合蛋白，Y盒是所有主要组织相容性复合体II类基因共同的启动子元件。由于14个碱基的Y元件反向含有CCAAT盒，因此我们被提示询问NF-Y是否实际上是CCAAT盒结合蛋白，以及它是否与先前描述的CCAAT结合因子CBP和CTF/NF-I有关。来自凝胶延迟、甲基化干扰、饱和突变和交叉竞争实验的数据明确地证明，NF-Y是一个完全不同的CCAAT盒结合实体。此外，这些实验还发现了第四种CCAAT结合蛋白，NF-Y*，它与胸苷激酶启动子相互作用。因此，显然存在识别CCAAT序列的多种因素；现在迫切需要了解这种多样性的功能意义。
NF-Y is a sequence-specific DNA-binding protein that recognizes the Y box, a promoter element common to all major histocompatibility complex class II genes. Since the 14-base Y element harbors a CCAAT box in reverse, we were prompted to ask whether NF-Y is actually a CCAAT box-binding protein and whether it is related to the previously described CCAAT-binding factors CBP and CTF/NF-I. Data from gel retardation, methylation interference, saturation mutagenesis, and cross-competition experiments establish definitively that NF-Y is an entirely distinct CCAAT box-binding entity. Moreover, these experiments have uncovered a fourth CCAAT-binding protein, NF-Y*, that interacts with the thymidine kinase promoter. Clearly, then, there exists a multiplicity of factors that recognize CCAAT sequences; it now becomes imperative to understand the functional significance of this multiplicity.