The porcine and lagomorph septal cartilages: Models for tissue engineering and morphologic cartilage research
The porcine and lagomorph septal cartilages: Models for tissue engineering and morphologic cartilage research
复制标题
DOI：
10.2500/105065801781543790
复制
发表时间：
2001-03-01
期刊：
影响因子：
--
通讯作者：
Nelson, JS
中科院分区：
文献类型：
--
作者：
Wong, BJF;Chao, KKH;Nelson, JS
使用先进的技术和仪器重建和修改面部软骨框架的兴趣正在迅速增长。尽管这种成熟的兴趣，没有动物模型已经建立，以提供形态学软骨组织具有相似的特征，以人类中隔在适当的数量。本研究的目的是表征猪和兔（兔）鼻中隔软骨组织。这两种模型与它们的人类对应物具有很大的相似性，并且提供了低成本、高容量和容易获得的大量软骨组织来源。我们提出了一种从这些物种中收获完整隔软骨的技术，并使用脉冲追踪放射性标记，流式细胞术和机械分析表征选择细胞代谢和物理特性。我们对关键组织特性的选择性评价确立了这些物种作为鼻中隔软骨手术的合适动物模型。
Interest in reconstruction and modification of the facial cartilaginous frameworks using advanced technology and instrumentation is growing rapidly. Despite this maturing interest, no animal model has been established to provide morphologic cartilage tissue with similar characteristics to human septum in suitable quantities. The objective of this study was to characterize porcine and lagomorph (rabbit) nasal septal cartilage tissue. Both models share great similarity with their human counterpart and provide a low-cost, high-volume, and easily obtained source of bulk cartilage tissue. We present a technique for harvesting intact septal cartilages from these species, and characterize select cellular metabolic, and physical properties using pulse-chase radiolabeling, flow cytometry, and mechanical analysis. Our selective evaluation of key tissue properties establishes these species as appropriate animal models for nasal septal cartilaginous surgery.