Novel sialic acid transporter of Haemophilus influenzae
Novel sialic acid transporter of Haemophilus influenzae
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DOI：
10.1128/iai.73.9.5291-5300.2005
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发表时间：
2005-09-01
影响因子：
3.1
通讯作者：
Apicella, MA
中科院分区：
文献类型：
--
作者：
Allen, S;Zaleski, A;Apicella, MA
无法分型的流感嗜血杆菌是一种条件致病菌，是儿童中耳炎、慢性支气管炎和慢性阻塞性肺病患者肺炎的常见病因。脂寡糖是H.流感，在微生物毒力和致病性中起重要作用。N-乙酰神经氨酸（唾液酸）可以作为末端非还原糖掺入脂寡糖中。虽然唾液酸掺入脂寡糖的途径已被了解，但在H.流感病毒的特征还有待鉴定。在本文中，我们证明了这种转运蛋白是一种新的糖转运蛋白的三方ATP-独立的周质转运蛋白家族。在缺乏这种转运蛋白的情况下，H.流感病毒无法将唾液酸结合到其脂低聚糖中，使该生物体在暴露于人血清时无法生存，并导致生物膜生长的活力降低。
Nontypeable Haemophilus influenzae is an opportunistic pathogen and a common cause of otitis media in children and of chronic bronchitis and pneumonia in patients with chronic obstructive pulmonary disease. The lipooligosaccharides, a major component of the outer membrane of H. influenzae, play an important role in microbial virulence and pathogenicity. N-Acetylneuraminic acid (sialic acid) can be incorporated into the lipooligosaccharides as a terminal nonreducing sugar. Although much of the pathway of siallic acid incorporation into lipooligosaccharides is understood, the transporter responsible for N-acetylneuraminic acid uptake in H. influenzae has yet to be characterized. In this paper we demonstrate that this transporter is a novel sugar transporter of the tripartite ATP-independent periplasmic transporter family. In the absence of this transporter, H. influenzae cannot incorporate siallic acid into its lipooligosaccharides, making the organism unable to survive when exposed to human serum and causing reduced viability in biofilm growth.