HOST-PATHOGEN INTERACTIONS .19. THE ENDOGENOUS ELICITOR, A FRAGMENT OF A PLANT-CELL WALL POLYSACCHARIDE THAT ELICITS PHYTOALEXIN ACCUMULATION IN SOYBEANS
HOST-PATHOGEN INTERACTIONS .19. THE ENDOGENOUS ELICITOR, A FRAGMENT OF A PLANT-CELL WALL POLYSACCHARIDE THAT ELICITS PHYTOALEXIN ACCUMULATION IN SOYBEANS
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DOI：
10.1104/pp.68.5.1161
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发表时间：
1981-01-01
期刊：
影响因子：
7.4
通讯作者：
ALBERSHEIM, P
中科院分区：
文献类型：
--
作者：
HAHN, MG;DARVILL, AG;ALBERSHEIM, P
从纯化的大豆(G.max[L.])细胞壁中溶解了植物抗毒素积累的激发子(内源激发子)。梅尔，cv.韦恩)通过用热水提取墙壁或使墙壁部分酸解。从大豆细胞壁获得的内源激发子与阴离子交换树脂结合。从树脂中释放的激发子活性物质含有富含半乳糖醛酸的低聚糖；也存在少量的鼠李糖和木糖。激发子活性片段中的半乳糖醛酸占优势，这表明激发子实际上是果胶多糖的片段。用高纯度的内切多聚半乳糖醛酸酶处理壁片会破坏它们诱导植物抗毒素积累的能力。显然，半乳糖醛酸是激发子活性壁碎片的重要组成部分。悬浮培养的烟草、梧桐树和小麦细胞壁上的内源激发子也被部分水解物溶解。
An elicitor of phytoalexin accumulation (endogenous elicitor) is solubilized from purified cell walls of soybean (G. max [L.] Merr., cv. Wayne) by extracting the walls with hot water or by subjecting the walls to partial acid hydrolysis. The endogenous elicitor obtained from soybean cell walls binds to an anion exchange resin. The elicitor-active material released from the resin contains oligosaccharides rich in galacturonic acid; small amounts of rhamnose and xylose are also present. The preponderance of galacturonic acid in the elicitor-active fragments suggests that the elicitor is, in fact, a fragment of a pectic polysaccharide. Treatment of the wall fragments with a highly purified endopolygalacturonase destroys their ability to elicit phytoalexin accumulation. Apparently, galacturonic acid is an essential constituent of the elicitor-active wall fragments. Endogenous elicitors were also solubilized by partial hydrolysis from cell walls of suspension-cultured tobacco, sycamore and wheat cells.