Preparation of 9Z-β-Carotene and 9Z-β-Carotene High-Loaded Nanostructured Lipid Carriers: Characterization and Storage Stability
Preparation of 9Z-β-Carotene and 9Z-β-Carotene High-Loaded Nanostructured Lipid Carriers: Characterization and Storage Stability
复制标题
9Z-β-胡萝卜素和 9Z-β-胡萝卜素高负载纳米结构脂质载体的制备：表征和储存稳定性
DOI：
10.1021/acs.jafc.0c02342
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发表时间：
2020
影响因子：
6.1
通讯作者：
Zhang Lianfu
中科院分区：
文献类型：
--
作者：
Yang Cheng;Yan Hongxiao;Jiang Xin;Xu Huaneng;Tsao Rong;Zhang Lianfu
制备了9 Z-β-胡萝卜素含量为25.3%的顺式（Z）-β-胡萝卜素纳米脂质载体。采用正交实验法确定了制备NLC的最佳条件：总脂浓度为9%（w/v），表面活性剂浓度为1.4%（w/v），固液比为3：1（w/w），均质压力为500 bar，均质3次。在此条件下制备的纳米脂质体包封率为95.64%，β-胡萝卜素含量为2.9mg/mL，显著高于其他文献报道的结果。β-胡萝卜素总含量高达53.3%，粒径为191 ± 6.46 nm，多分散指数为0.2 ± 0.03。储存稳定性结果表明，负载β-胡萝卜素的NLC在低温（4 °C）下在pH 3.5-7.5下储存21天期间稳定9 Z-β-胡萝卜素和总β-胡萝卜素免于渗漏和降解，尤其是对于更具生物活性的9 Z-β-胡萝卜素。该技术提高了9 Z-β-胡萝卜素的比例、负载能力、水溶性和β-胡萝卜素NLC的生物可及性，为β-胡萝卜素在功能性食品或饮料以及营养制剂中的应用提供了有效的策略。
Cis(Z)-β-carotenes with 25.3% 9Z-β-carotene were prepared for nanostructured lipid carriers (NLCs). The optimal conditions for NLC preparation using an orthogonal experimental method were as follows: the total lipid concentration was 9% (w/v), the surfactant concentration was 1.4% (w/v), the solid to liquid lipid ratio was 3:1 (w/w), and the homogenization pressure was set at 500 bar for three cycles. Under these conditions, the encapsulation efficiency (%) of the NLC was 95.64%, and the total β-carotene in NLCs was 2.9 mg/mL, which was significantly higher than those reported by others. The proportion of totalZ-β-carotenes was as high as 53.3%, the particle size was 191 ± 6.46 nm, and the polydispersity index was 0.2 ± 0.03. Storage stability results indicated that the β-carotene-loaded NLC stabilizes both 9Z-β-carotene and total β-carotene from leakage and degradation during 21 days of storage at pH 3.5–7.5 at low temperatures (4 °C), especially for the more bioactive 9Z-β-carotene. The technique with an improved ratio of 9Z-β-carotene, loading capacity, water solubility, and bioaccessibility of the β-carotene NLC provides an effective strategy for β-carotene applications in functional foods or beverages and in nutraceutical preparations.