The Clothes Make the mRNA: Past and Present Trends in mRNP Fashion.
The Clothes Make the mRNA: Past and Present Trends in mRNP Fashion.
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DOI：
10.1146/annurev-biochem-080111-092106
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发表时间：
2015
影响因子：
16.6
通讯作者：
Moore MJ
中科院分区：
文献类型：
--
作者：
Singh G;Pratt G;Yeo GW;Moore MJ
在它们的整个生命周期中，信使RNA（mRNA）与蛋白质结合形成核糖核蛋白（mRNP）。自从40多年前发现第一个mRNP组分以来，所谓的mRNA相互作用组现在包含超过1，000种蛋白质。这些蛋白质以不同的亲和力和化学计量以无数方式结合mRNA，其中许多在转录和前体mRNA（pre-mRNA）加工期间以高度有序的过程组装到新生RNA上。在全转录组研究中观察到的主要mRNP蛋白的非随机分布使我们提出mRNP被组织成三个主要结构域，松散地对应于5′非翻译区（UTR）、开放阅读框和3′ UTR。从细胞核移动到细胞质，mRNP经历广泛的重塑，因为它们首先受到核孔复合物的作用，然后受到核糖体的作用。当不被主动翻译时，细胞质mRNP可以组装成大的多mRNP组件或被永久分解和降解。在这篇综述中，我们的目标是让读者对过去和当前的真核mRNP研究有一个透彻的了解。
Throughout their lifetimes, messenger RNAs (mRNAs) associate with proteins to form ribonucleoproteins (mRNPs). Since the discovery of the first mRNP component more than 40 years ago, what is known as the mRNA interactome now comprises >1,000 proteins. These proteins bind mRNAs in myriad ways with varying affinities and stoichiometries, with many assembling onto nascent RNAs in a highly ordered process during transcription and precursor mRNA (pre-mRNA) processing. The nonrandom distribution of major mRNP proteins observed in transcriptome-wide studies leads us to propose that mRNPs are organized into three major domains loosely corresponding to 5′ untranslated regions (UTRs), open reading frames, and 3′ UTRs. Moving from the nucleus to the cytoplasm, mRNPs undergo extensive remodeling as they are first acted upon by the nuclear pore complex and then by the ribosome. When not being actively translated, cytoplasmic mRNPs can assemble into large multi-mRNP assemblies or be permanently disassembled and degraded. In this review, we aim to give the reader a thorough understanding of past and current eukaryotic mRNP research.