Astrocytes specifically remove surface-adsorbed fibrinogen and locally express chondroitin sulfate proteoglycans.
Astrocytes specifically remove surface-adsorbed fibrinogen and locally express chondroitin sulfate proteoglycans.
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DOI：
10.1016/j.actbio.2013.02.047
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发表时间：
2013-07
影响因子：
9.7
通讯作者：
Hlady, Vladimir
中科院分区：
文献类型：
--
作者：
Hsiao, Tony W.;Swarup, Vimal P.;Kuberan, Balagurunathan;Tresco, Patrick A.;Hlady, Vladimir
表面吸附纤维蛋白原（FBG）被粘附的星形胶质细胞识别，并通过两相去除过程从底物中去除。细胞从二元蛋白的表面模式（FBG +层粘连蛋白或FBG +白蛋白）中去除吸附的FBG，而留下另一种蛋白质。星形胶质细胞在纤维蛋白原刺激位点优先表达硫酸软骨素蛋白多糖（CSPG）；然而，作为FBG表面覆盖的函数，总体CSPG产量没有差异。星形胶质细胞去除FBG也不依赖于基于转化生长因子β (TGF-β)受体的信号传导，因为在TGF-β受体激酶抑制剂SB 431542存在的情况下，细胞维持CSPG的产生。该抑制剂降低了CSPG的表达，但没有完全消除CSPG。由于血液接触和随后的FBG吸附在神经植入中是不可避免的，研究结果表明，由于星形胶质细胞特异性识别被吸附的FBG，植入物吸附的FBG可能有助于植入物周围的反应性星形胶质细胞形成。
Surface-adsorbed fibrinogen (FBG) was recognized by adhering astrocytes and removed from the substrates in vitro by a two-phase removal process. The cells removed adsorbed FBG from binary proteins surface patterns (FBG + laminin, or FBG + albumin) while leaving the other protein behind. Astrocytes preferentially expressed chondroitin sulfate proteoglycan (CSPG) at the loci of fibrinogen stimuli; however no differences in overall CSPG production as a function of FBG surface coverage were identified. Removal of FBG by astrocytes was also found to be independent of transforming growth factor type β (TGF-β) receptor based signaling as cells maintained CSPG production in the presence of TGF-β receptor kinase inhibitor, SB 431542. The inhibitor decreased CSPG expression, but did not abolicsh it entirely. Because blood contact and subsequent FBG adsorption are unavoidable in neural implantations, the results indicate that implant-adsorbed FBG may contribute to reactive astrogliosis around the implant as astrocytes specifically recognize adsorbed FBG.
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DOI：
10.1083/jcb.85.3.890
发表时间：
1980-06
期刊：
The Journal of cell biology
影响因子：
--
作者：
McCarthy KD;de Vellis J
通讯作者：
de Vellis J
影响因子：
2.2
作者：
Friedman, Alon;Kaufer, Daniela;Heinemann, Uwe
通讯作者：
Heinemann, Uwe
DOI：
10.1523/jneurosci.0137-10.2010
发表时间：
2010-04-28
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Schachtrup C;Ryu JK;Helmrick MJ;Vagena E;Galanakis DK;Degen JL;Margolis RU;Akassoglou K
通讯作者：
Akassoglou K
影响因子：
3.5
作者：
Lagord, C;Berry, M;Logan, A
通讯作者：
Logan, A
影响因子：
2.9
作者：
OCALLAGHAN, JP;MILLER, DB;REINHARD, JF
通讯作者：
REINHARD, JF