Near-infrared fluorescent type II quantum dots for sentinel lymph node mapping
Near-infrared fluorescent type II quantum dots for sentinel lymph node mapping
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DOI：
10.1038/nbt920
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发表时间：
2004-01-01
影响因子：
46.9
通讯作者：
Frangioni, JV
中科院分区：
文献类型：
--
作者：
Kim, S;Lim, YT;Frangioni, JV
近红外或红外光子的使用是活体组织生物医学成像的一种有前途的方法（1）。该技术通常需要具有流体动力学直径、吸收、量子产率和稳定性的组合的外源性造影剂，这对于常规有机荧光团是不可能的。在这里，我们表明，II型2，3量子点的荧光发射可以调谐到近红外，同时保持吸收截面，并且多齿膦涂层使它们在血清中可溶，分散和稳定。然后，我们证明这些量子点允许在完整的图像引导下在大型动物中进行重大癌症手术，即前哨淋巴结映射（4-6）。仅注射400 pmol近红外量子点，即可使用仅5 mW/cm的激发注量率轻松对1 cm深的前哨淋巴结进行真实的成像（2）。总之，在这项研究中提出的化学，光学和体内数据证明了近红外量子点的生物医学成像的潜力。
The use of near-infrared or infrared photons is a promising approach for biomedical imaging in living tissue(1). This technology often requires exogenous contrast agents with combinations of hydrodynamic diameter, absorption, quantum yield and stability that are not possible with conventional organic fluorophores. Here we show that the fluorescence emission of type II2,3 quantum dots can be tuned into the near infrared while preserving absorption cross-section, and that a polydentate phosphine coating renders them soluble, disperse and stable in serum. We then demonstrate that these quantum dots allow a major cancer surgery, sentinel lymph node mapping(4-6), to be performed in large animals under complete image guidance. Injection of only 400 pmol of near-infrared quantum dots permits sentinel lymph nodes 1 cm deep to be imaged easily in real time using excitation fluence rates of only 5 mW/cm(2). Taken together, the chemical, optical and in vivo data presented in this study demonstrate the potential of near-infrared quantum dots for biomedical imaging.