Surface morphology of trypsinized human cells in vitro.
Surface morphology of trypsinized human cells in vitro.
复制标题
体外胰蛋白酶处理的人体细胞的表面形态。
DOI：
10.1016/0014-4827(71)90688-4
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发表时间：
1971
影响因子：
3.7
通讯作者：
P. Todd
中科院分区：
文献类型：
--
作者：
H. Dalen;P. Todd
在单层培养物的胰蛋白酶处理过程中，通过相差和扫描电子显微镜观察常人肝细胞。扁平的间期细胞被形成球形结构，留下细长的细胞质突起，称为胰蛋白酶消化回缩原纤维，其类似于有丝分裂回缩原纤维，但与有丝分裂回缩原纤维不同，形成得更快（1-2分钟）。酶处理揭示的另一种长微延伸是附着原纤维，它们是处于张力下的细胞间细胞质线。从正常间期细胞表面突出的短微延伸在暴露于 0.03% 胰蛋白酶溶液的前 5 分钟内消失。
Chang human liver cells were observed by phase contrast and scanning electron microscopy during trypsin treatment of monolayer cultures. The flattened interphase cell was caused to round up into a spherical structure leaving behind long slender cytoplasmic processes, named trypsinization retraction fibrils, which resemble the mitotic retraction fibrils but differ from these by being formed more rapidly (1–2 min). Another type of long microextensions revealed by the enzymatic treatment are the attachment fibrils, which are intercellular cytoplasmic strings under tension. The short microextensions projecting from the surface of the normal interphase cells disappear during the first 5 min of exposure to 0.03% trypsin solution.