Effect of haptoglobin on the metabolism of vitamin C
Effect of haptoglobin on the metabolism of vitamin C
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DOI：
10.1093/ajcn/66.3.606
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发表时间：
1997-09-01
影响因子：
7.1
通讯作者：
Ouyang, J
中科院分区：
文献类型：
--
作者：
Langlois, MR;Delanghe, JR;Ouyang, J
结合珠蛋白是一种血红蛋白结合抗氧化剂，具有三种类型的遗传多态性：Hp 1-1，Hp 2-1和Hp 2-2。Hp 2-2型与动脉粥样硬化的风险增加有关。我们研究了维生素C代谢在体内和体外根据触珠蛋白类型在一个研究组的135名健康志愿者。血清维生素C浓度与结合珠蛋白类型有关，Hp 2-2型血清维生素C浓度最低（P < 0.01）。肾阈值L-抗坏血酸是在正常范围内，代谢为草酸盐之间没有不同的触珠蛋白型组。其他内源性抗氧化剂（尿酸，胆红素，白蛋白，血浆铜蓝蛋白和总抗氧化状态）的血清浓度在触珠蛋白型组之间没有差异。体外实验表明，Hp 2-2型血中L-抗坏血酸的稳定性较低（P < 0.01）。L-抗坏血酸体外消耗与结合珠蛋白浓度呈负相关（r = -0.738）。本研究的结果表明，Hp 2-2携带者中的L-抗坏血酸氧化速率较高，因为它们对血红蛋白-铁驱动的过氧化的保护较少。
Haptoglobin is a hemoglobin-binding antioxidant showing a genetic polymorphism with three types: Hp 1-1, Hp 2-1, and Hp 2-2. The Hp 2-2 type has been associated with an increased risk of atherosclerosis. We investigated vitamin C metabolism in vivo and in vitro according to haptoglobin type in a study group of 135 healthy volunteers. Serum vitamin C concentrations were associated with haptoglobin type, showing lowest values in serum from Hp 2-2 subjects (P < 0.01). Renal threshold for L-ascorbic acid was within the normal range and metabolization to oxalate was not different among haptoglobin-type groups. Serum concentrations of other endogenous antioxidants (uric acid, bilirubin, albumin, ceruloplasmin, and total antioxidative status) were not different among haptoglobin-type groups. In vitro experiments showed a lower stability of L-ascorbic acid in blood from subjects with the Hp 2-2 type (P < 0.01). L-Ascorbic acid depletion in vitro was inversely related to haptoglobin concentration (r = -0.738). The results of this study indicate a higher rate of L-ascorbic acid oxidation in Hp 2-2 carriers because they have less protection against hemoglobin-iron driven peroxidation.