Short-term exposure to low concentrations of the synthetic androgen methyltestosterone affects vitellogenin and steroid levels in adult male zebrafish (Danio rerio).
Short-term exposure to low concentrations of the synthetic androgen methyltestosterone affects vitellogenin and steroid levels in adult male zebrafish (Danio rerio).
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DOI：
10.1016/j.aquatox.2005.10.008
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发表时间：
2006-03
影响因子：
4.5
通讯作者：
L. Andersen;R. Goto-Kazeto;J. Trant;J. P. Nash;B. Korsgaard;P. Bjerregaard
中科院分区：
文献类型：
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作者：
L. Andersen;R. Goto-Kazeto;J. Trant;J. P. Nash;B. Korsgaard;P. Bjerregaard
研究了甲基睾酮（MT）对成年雄性斑马鱼内分泌系统的短期影响。雄性分别暴露于0、4.5、6.6、8.5、19.8、35.9、62.3ng MT/l和炔雌醇（EE2） （26.4ng/l） 7 d。我们分析了可能受内分泌干扰物影响的几个生理终点，特别是卵黄原蛋白（VTG）浓度、雌二醇（E2）、睾酮(T)和11-酮睾酮（KT）含量、脑芳香化酶活性和睾丸中CYP19A1和CYP19A2的基因表达。与溶剂对照组相比，暴露于最低MT浓度（4.5ng MT/l）和EE2使VTG浓度显著增加。暴露于较高浓度的MT并没有增加VTG水平。内源KT和T水平随MT暴露呈浓度依赖性显著降低，最低有效浓度分别为6.4和8.5ng MT/l。与溶剂对照组相比，EE2也显著抑制了KT和T的水平。在一些MT组中发现内源性E2水平显著降低，但不可能区分简单的浓度-反应关系。MT和EE2对脑组织芳香化酶活性及睾丸CYP19A1和CYP19A2基因表达均无影响。结果表明，MT等雄激素即使在非常低的浓度下也能起到内分泌干扰物的作用。
Short-term effects of methyltestosterone (MT) on the endocrine system of adult male zebrafish (Danio rerio) were examined. Males were exposed to 0, 4.5, 6.6, 8.5, 19.8, 35.9, 62.3ng MT/l and ethinylestradiol (EE2) (26.4ng/l) for 7 days. Several physiological endpoints that may be affected by endocrine disrupters were analysed, specifically vitellogenin (VTG) concentration, estradiol (E2), testosterone (T), and 11-ketotestosterone (KT) content, brain aromatase activity and gene expression of CYP19A1 and CYP19A2 in the testis. Exposure to the lowest MT concentration (4.5ng MT/l), and the EE2 increased the concentration of VTG significantly compared to solvent control group. Exposure to higher concentrations of MT did not increase VTG levels. Endogenous KT and T levels decreased significantly in a concentration-dependent manner in response to the MT exposure and the lowest effective concentrations were 6.4 and 8.5ng MT/l, respectively. The levels of KT and T were also significantly suppressed by EE2 when compared to the solvent control group. Significant decreases in endogenous E2 levels were found in some MT groups but it was not possible to distinguish a simple concentration–response relationship. No effects of MT or EE2 on the brain aromatase activity or on testicular gene expression of CYP19A1 and CYP19A2 were detected. The results show that androgens such as MT can act as endocrine disrupters even at very low concentrations.