Selective recovery of microalgae from diverse habitats using "phyto-specific" 16S rDNA primers
Selective recovery of microalgae from diverse habitats using "phyto-specific" 16S rDNA primers
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DOI：
10.1111/j.1529-8817.2007.00350.x
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发表时间：
2007-06-01
影响因子：
2.9
通讯作者：
Stiller, John W.
中科院分区：
文献类型：
--
作者：
Betournay, Scott;Marsh, Amanda C.;Stiller, John W.
环境聚合酶链式反应是调查水生微藻的常用工具；然而，通常使用的通用引物并不是针对浮游植物的，通常会高频地回收非光合作用的细菌。利用16S rDNA“植物特异性”引物，我们能够选择性地从几个混合的水生样品中扩增出光合作用物种的序列，即使在存在大量非光合作用微生物的情况下也是如此。我们鉴定了来自三种不同栖息地的21种微藻序列：弗吉尼亚州的盐沼、北卡罗来纳州的河流盆地和阿拉斯加的海冰。相比之下，通用的16S引物从一些相同的样本中恢复了大部分非光合作用的有机体。我们的结果表明，浮游植物特异性引物在选择性地扩增混合环境样品中广泛多样性的微藻方面是有效的，因此可以减少外来物种的噪音，这些外来物种往往主导着水生样品的分子调查。
Environmental PCR is a common tool for surveying aquatic microalgae; however, universal primers generally employed are not specific to phytoplankton and typically recover nonphotosynthetic bacteria at high frequencies. Using a 16S rDNA "phyto-specific" primer, we were able to selectively amplify sequences of photosynthetic species from several mixed aquatic samples, even when large numbers of nonphotosynthetic microorganisms were present. We identified 21 microalgal sequences from three different habitats: salt marshes in Virginia, river basins in North Carolina, and sea ice in Alaska. In contrast, universal 16S primers recovered a majority of nonphotosynthetic organisms from some of the same samples. Our results indicate that phytoplankton-specific primers are efficient in selectively amplifying a broad diversity of microalgae in mixed environmental samples and, therefore, can reduce the noise from extraneous species that often dominates molecular surveys of aquatic samples.