Collagen degradation in aged/photodamaged skin in vivo and after exposure to matrix metalloproteinase-1 in vitro
Collagen degradation in aged/photodamaged skin in vivo and after exposure to matrix metalloproteinase-1 in vitro
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DOI：
10.1046/j.1523-1747.2003.12148.x
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发表时间：
2003-05-01
影响因子：
6.5
通讯作者：
Voorhees, JJ
中科院分区：
文献类型：
--
作者：
Fligiel, SEG;Varani, J;Voorhees, JJ
采用生化和超微结构方法检测光损伤皮肤中胶原蛋白的变化。观察到广泛的胶原碎裂，碎裂的胶原成块，成纤维细胞与受损的基质相互作用。类似的，尽管范围较小，胶原蛋白损伤也在防晒皮肤中观察到--80岁或以上的人(自然老化的皮肤)。相比之下，年轻人(18-29岁)的防晒皮肤几乎没有损伤。胶原纤维分布均匀。间质成纤维细胞包埋在胶原基质中，与完整的胶原纤维紧密贴合。在其他研究中，I型胶原的三维晶格在体外暴露于基质金属蛋白酶-1(间质胶原酶)，并检查生化和超微结构的变化。在酶处理导致30-40%的胶原分子碎裂的条件下，晶格显示受损基质的胶原碎裂和聚集。最近的研究表明，在体外，成纤维细胞与胶原片段接触时会失去前胶原的产生。这项研究表明，在衰老和光损伤的皮肤中，体内胶原结构也发生了类似的变化。我们认为，体内的胶原碎裂可能是光损伤皮肤胶原合成丧失的基础，在较小程度上，也可能是衰老皮肤的胶原合成丧失的原因。
Biochemical and ultrastructural approaches were used to assess collagen changes in photodamaged skin. Extensive collagen fragmentation, clumping of the fragmented collagen, and interaction of fibroblasts with the damaged matrix were observed. Similar, though less extensive, collagen damage was also observed in sun-protected skin-individuals aged 80 y or older (naturally aged skin). In comparison, sun-protected skin from young individuals (18-29 y of age) demonstrated little damage. A uniform distribution of collagen fibrils was seen. Interstitial fibroblasts were embedded in the collagen matrix and in close apposition with intact collagen fibrils. In additional studies, three-dimensional lattices of type I collagen were exposed in vitro to matrix metalloproteinase-1 (interstitial collagenase), and examined for biochemical and ultrastructural alterations. Under conditions in which enzyme treatment produced fragmentation in 30-40% of the collagen molecules, the lattices demonstrated collagen fragmentation and clumping of the damaged matrix. Recent studies have demonstrated a loss of procollagen production by fibroblasts in contact with collagen fragments in vitro. This study demonstrates similar changes in collagen structure in vivo in aged and photodamaged skin. We suggest that collagen fragmentation in vivo could underlie the loss of collagen synthesis in photodamaged skin and, to a lesser extent perhaps, in aged skin.