Biomarkers for moni- toring profluthrin exposure : Urinary excretion kinetics of profluthrin me- tabolites in rats
Biomarkers for moni- toring profluthrin exposure : Urinary excretion kinetics of profluthrin me- tabolites in rats
复制标题
监测丙氟菊酯暴露的生物标志物：大鼠丙氟菊酯代谢物的尿排泄动力学
DOI：
10.1016/j.etap.2014.03.019
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Toshiaki Yoshida
中科院分区：
文献类型：
--
作者：
Tsuruya K;Fukuma S;Wakita T;Ninomiya T;Nagata M;Yoshida H;Fujimi S;Kiyohara Y;Kitazono T;Uchida K;Shirota T;Akizawa T;Akiba T;Saito A;Fukuhara S.;Toshiaki Yoshida
近年来，拟除虫菊酯[(2，3，5，6-四氟-4-甲基苯基)甲基2，2-dimethyl-3-(prop-1-enyl)cyclopropane-1-carboxylate]]被广泛用作室内防蛾驱避剂。为了寻找适合作为氟氰菊酯暴露生物标志物的尿代谢物，对其代谢产物在大鼠体内的排泄动力学进行了研究。给大鼠单次腹腔注射氟氰菊酯(26~400 mg/kg体重)，定期采集尿液。用气相色谱-质谱法测定了4-甲基-2，3，5，6-四氟苯甲醇(CH3-FB-Al)、4-羟甲基-2，3，5，6-四氟苯甲醇、4-甲基-2，3，5，6-四氟苯甲酸和2，2-dimethyl-3-(1-propenyl)-cyclopropanecarboxylic酸等4种主要氟氰菊酯代谢物。各代谢物的动力学评价通过尿排泄率-时间曲线的矩分析来实现。除MCA外，这三种代谢物的尿排泄量估计在较大的暴露范围内与氟氰菊酯的吸收量成正比。尿CH3-FB-Al被认为是监测氟氰菊酯的最佳生物标志物。
Recently, a pyrethroid profluthrin [(2,3,5,6-tetrafluoro-4-methylphenyl)methyl 2,2-dimethyl-3-(prop-1-enyl)cyclopropane-1-carboxylate] is widely used as mothproof repellents in indoors. The urinary excretion kinetics of its metabolites was examined in rats to search for urinary metabolites suitable as biomarkers of profluthrin exposure in the general population. A single dose (26–400 mg/kg body weight) of profluthrin was administered intraperitoneally to the rats, and then their urine was collected periodically. Four major profluthrin metabolites, 4-methyl-2,3,5,6-tetrafluorobenzyl alcohol (CH3-FB-Al), 4-hydroxymethyl-2,3,5,6-tetrafluorobenzyl alcohol, 4-methyl-2,3,5,6-tetrafluorobenzoic acid and 2,2-dimethyl-3-(1-propenyl)-cyclopropanecarboxylic acid (MCA) were determined in the urine samples by gas chromatography/mass spectrometry. The kinetic evaluation for each metabolite was achieved by moment analysis of the urinary excretion rateversustime curve. The urinary excretion amounts of the three metabolites, expect for MCA, were estimated to be proportional to the amounts of absorbed profluthrin over a wide exposure range. Urinary CH3-FB-Al was considered to be an optimal biomarker for monitoring of profluthrin.