Repair of DNA loops involves DNA-mismatch and nucleotide-excision repair proteins
Repair of DNA loops involves DNA-mismatch and nucleotide-excision repair proteins
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DOI：
10.1038/43225
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发表时间：
1997-06-26
期刊：
影响因子：
64.8
通讯作者：
Petes, TD
中科院分区：
文献类型：
--
作者：
Kirkpatrick, DT;Petes, TD
许多酶识别和修复DNA损伤（1）。DNA错配修复系统纠正碱基错配和小环，而核苷酸切除修复系统去除嘧啶二聚体和其他螺旋扭曲病变。具有错配或环的DNA分子可以作为减数分裂重组期间异源双链体形成的结果而出现（2）。在酿酒酵母中，错配的修复导致基因转换或恢复，而错配修复失败导致减数分裂后分离（PMS）（图1）。基因转换与PMS事件的比率反映了DNA修复的效率（3，4）。通过研究PMS模式的酵母菌株杂合子突变等位基因与26个碱基对插入，我们发现，26个碱基的环修复涉及Msh2（DNA错配修复蛋白）和Rad1（核苷酸切除修复所需的蛋白质）。
A number of enzymes recognize and repair DNA lesions(1). The DNA-mismatch repair system corrects base-base mismatches and small loops, whereas the nucleotide-excision repair systems removes pyrimidine dimers and other helix-distorting lesions. DNA molecules with mismatches or loops can arise as a consequence of heteroduplex formation during meiotic recombination(2). In the yeast Saccharomyces cerevisiae, repair of mismatches results in gene conversion or restoration, and failure to repair the mismatch results in post-meiotic segregation (PMS) (Fig. 1). The ratio of gene-conversion to PMS events reflects the efficiency of DNA repair(3,4). By examining the PMS patterns in yeast strains heterozygous for a mutant allele with a 26-base-pair insertion, we find that the repair of 26-base loops involves Msh2 (a DNA-mismatch repair protein) and Rad1 (a protein required for nucleotide-excision repair).