Mutation analysis of DC-SIGN promoter in chronic hepatitis B patients.
Mutation analysis of DC-SIGN promoter in chronic hepatitis B patients.
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DOI：
10.3969/j.issn.1672-7347.2011.11.004
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发表时间：
2011-11
期刊：
影响因子：
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通讯作者：
Li Chen;Congzhi Li;Xiujuan Meng;P. Zhu;D. Tan
中科院分区：
文献类型：
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作者：
Li Chen;Congzhi Li;Xiujuan Meng;P. Zhu;D. Tan
目的 探讨慢性乙型肝炎（CHB）患者和乙型肝炎病毒（HBV）感染者的DC-SIGN启动子区是否存在突变，并探讨树突状细胞特异性细胞间粘附分子3-抓取非整合素（DC-SIGN）启动子区突变与HBV的关系。方法 研究人群由两组组成：47 名慢性乙型肝炎患者和 20 名既往感染过乙肝病毒的健康人。通过PCR、单链构象多态性和异源双链分析、克隆、测序和比对已发表的DC-SIGN启动子序列来检测DC-SIGN启动子区域的突变。结果 HBV感染者DC-SIGN启动子区出现特征性突变。在CHB患者的DC-SIGN启动子区中，在-139、-142、-222和-336位点发现了4个热点突变，而在既往感染过HBV的健康人中仅观察到了位于-139位的1个点突变。 11名慢性乙型肝炎患者（23.40%）出现了先前感染过乙型肝炎的健康人中不存在的-336C。 -139T 在既往感染过 HBV 的健康人中出现的频率 (100%) 远高于 CHB 患者 (34.04%)。结论 在 DC-SIGN 启动子区域，-336C 可能是发生 CHB 的遗传危险因素，但 -139T 可能与预防 HBV 相关。
OBJECTIVE To investigate whether there is mutation in DC-SIGN promoter region in patients with chronic hepatitis B (CHB) and healthy persons previously infected with hepatitis B virus (HBV) and to explore the relationship between the mutation in dendritic cell-specific intercellular adhension molecule-3-grabbing nonintegrin (DC-SIGN) promoter region and HBV. METHODS The studied population was composed of two cohorts: 47 CHB patients and 20 healthy persons previously infected with HBV. The mutation in DC-SIGN promoter region was detected with PCR, single-stranded conformational polymorphism and heteroduplex analysis, cloning, sequencing and aligning the published DC-SIGN promoter sequence. RESULTS The characteristic mutation within DC-SIGN promoter region in HBV infected individuals was observed. In the DC-SIGN promoter region, 4 hot spot mutations located in positions -139, -142, -222, and -336 were observed in the CHB patients, but only 1 spot mutation located in position -139 was observed in the healthy persons previously infected with HBV. The -336C which was absent in the healthy persons previously infected with HBV was shown in 11 CHB patients (23.40%). The -139T was far more frequent in the healthy persons previously infected with HBV (100%) than in the CHB patients (34.04%). CONCLUSION In the DC-SIGN promoter region, -336C may be a genetic risk factor for developing CHB, but -139T may be associated with protection against HBV.