Germinal center B cells present antigen obtained in vivo to T cells in vitro and stimulate mixed lymphocyte reactions.
Germinal center B cells present antigen obtained in vivo to T cells in vitro and stimulate mixed lymphocyte reactions.
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生发中心B细胞将体内获得的抗原呈递给体外的T细胞，并刺激混合淋巴细胞反应。
DOI：
10.1007/978-1-4684-5535-9_132
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发表时间：
1988
影响因子：
--
通讯作者：
Tew,JG
中科院分区：
文献类型：
--
作者：
Kosco,MH;Monfalcone,AP;Szakal,AK;Tew,JG
在二次免疫后不久，在超微结构研究中观察到生发中心（GC） B细胞以免疫复合物的形式从滤泡树突状细胞（fdc）中获得抗原(1)。这种抗原似乎被GC B细胞降解，并可能被加工呈递给T细胞。本研究旨在确定GC B细胞是否能够加工并将从fdcsinsinv体内获得的抗原呈递到合适的体外T细胞。此外，在原发性混合淋巴细胞反应（MLR）中评估GC B细胞对T细胞的刺激能力。本文的研究结果表明，GC B细胞在体内获得的抗原可以被加工并提呈给T细胞。GC B细胞与T细胞相互作用的能力表明，它们是同种异体T细胞的有效刺激物。相反，在相同的条件下，小的高密度B细胞对负刺激弱。此外，GC B细胞甚至能够刺激可检测到的同基因MLRs。这些数据表明，在体内，GC B细胞能够与T细胞相互作用，进而诱导生发中心反应所需的T细胞帮助，产生B记忆细胞，并影响与二抗反应相关的高滴度抗体。
Shortly after secondary immunization, germinal center (GC) B cells have been observed in ultrastructural studies to obtain antigen from follicular dendritic cells (FDCs) in the form of immune complexes (1). This antigen appears to be degraded by GC B cells and may be processed for presentation to T cells. The present study was undertaken to determine if GC B cells can process and present antigen obtained from FDCsinvivoto appropriate T cells invitro. In addition, the stimulatory capacity of GC B cells on T cells was evaluated in primary mixed lymphocyte reactions (MLR). The findings presented here demonstrate that antigen obtained invivoby GC B cells can be processed and presented to T cells. The ability of GC B cells to interact with T cells is indicated by the fact that they served as potent stimulators of allogeneic T cells. In contrast, the small high density (HD) B cells are weak to negative stimulators under the same conditions. In addition, the GC B cells were even able to stimulate detectable syngeneic MLRs. These data suggest that invivo, GC B cells are capable of interacting with T cells, in turn inducing the T cell help needed for the germinal center reaction, generating B memory cells, and influencing the high titers of antibody associated with the secondary antibody response.