The natural occurrence of two distinct begomoviruses associated with DNAβ and a recombinant DNA in a tomato plant from Indonesia
The natural occurrence of two distinct begomoviruses associated with DNAβ and a recombinant DNA in a tomato plant from Indonesia
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DOI：
10.1094/phyto-96-0517
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发表时间：
2006-05-01
期刊：
影响因子：
3.2
通讯作者：
Ikegami, M
中科院分区：
文献类型：
--
作者：
Kon, T;Hidayat, SH;Ikegami, M
从印度尼西亚一株具有卷叶症状的番茄植株中克隆出两种begomovirus （Java virus-1和Java virus-2）、两种卫星DNA β （DNA β 01和DNA β 02）和一种重组DNA (recDNA)，并对这些卫星DNA在begomvirus病发病机制中的作用进行了研究。这两种病毒的基因组组织与其他东半球单株begomovirus相似。与其他begomovirus的序列比较表明，Java病毒-1是一种新发现的病毒，并提出了番茄卷叶Java病毒（ToLCJAV）的名称。Java病毒-2是Ageratum yellow vein virus （AYVV-[Java]）的一个毒株。单独使用ToLCJAV或AYVV-[Java]不会引起番茄叶片卷曲症状。然而。在DNA β 02的存在下ToLCJAV和AYVV-[爪哇]均可诱导番茄叶片卷曲。在DNA β 01存在的情况下，这些病毒在番茄植株上引起轻微的叶片卷曲症状。recDNA有一个嵌合序列。它源于tolcjava， AYVV-[Java]之间的重组。DNA β 01和DNA β 02；它只有在存在AYVV-[Java]的情况下才能在番茄植株中复制。
Two begomoviruses (Java virus-1 and Java virus-2), two satellite DNA beta (DNA beta 01 and DNA beta 02), and a recombinant DNA (recDNA) were cloned from a single tomato plant from Indonesia with leaf Curl Symptoms, and the role of these satellite DNAs in the etiology of begomovirus disease was investigated. The genome organizations of the two viruses were similar to those of other Old World monopartite begomoviruses. Comparison of the sequences with other begomoviruses revealed that Java virus-1 was a newly described virus for which the name Tomato leaf curl Java virus (ToLCJAV) is proposed. Java virus-2 was a strain of Ageratum yellow vein virus (AYVV) (AYVV-[Java]). ToLCJAV or AYVV-[Java] alone did not induce leaf curl symptoms in tomato plants. However. in the presence of DNA beta 02. both ToLCJAV and AYVV-[Java] induced leaf curl symptoms in tomato plants. In the presence of DNA beta 01, these viruses induced mild leaf curl symptoms in tomato plants. The recDNA had a chimeric sequence. which arose from recombination among ToLCJAV, AYVV-[Java]. DNA beta 01 and DNA beta 02; it was replicated only in the presence of AYVV-[Java] in tomato plants.