Beneficial effect of D-allose for isolated islet culture prior to islet transplantation.
Beneficial effect of D-allose for isolated islet culture prior to islet transplantation.
复制标题
D-阿洛糖对胰岛移植前分离胰岛培养的有益作用。
DOI：
10.1002/jhbp.298
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Kashiwagi H
中科院分区：
文献类型：
--
作者：
Ogawa;H.;Hatakeyama;Y.;Takahoashi;K.;Tominaga;M.;Kimura;H.;Ohta;T.;Kashiwagi H
背景隔离期间受损胰岛的移植前修复仍有待解决。在这项研究中，我们研究了D-阿洛糖对胰岛的影响，从大鼠胰腺分离胰岛前胰岛transplantation.MethodsRat胰岛分离新鲜胰腺培养过夜罗斯威尔公园纪念研究所1640解决方案中的情况下（组1）或存在（组2）D-阿洛糖。观察胰岛素刺激指数和胰岛移植治疗糖尿病裸鼠的治愈率。我们还测定了丙二醛水平和caspase 3活性的胰岛细胞培养过夜后的氧化应激和凋亡的评估。第2组的刺激指数显著高于第1组。移植后第2组的治愈率高于第1组，尤其是第1周。第2组的丙二醛水平显著低于第1组。但两组的caspase 3活性没有差异。结论移植前用D-阿洛糖处理离体胰岛培养物可恢复胰岛功能，提高移植成功率。这项研究的结果表明，D-阿洛糖通过其抗氧化活性改善受损胰岛的功能。
BackgroundPretransplant restoration of islets damaged during isolation remains to be solved. In this study, we examined the effect of D‐allose on islets isolated from rat pancreata prior to islet transplantation.MethodsRat islets isolated from fresh pancreata were cultured overnight in Roswell Park Memorial Institute 1640 solution in the absence (group 1) or presence (group 2) of D‐allose. Then we assessed stimulation index of insulin, and cure rate after islet transplantation to diabetic nude mice. We also measured malondialdehyde level and caspase 3 activity of islets after the overnight culture for assessment of the oxidative stress and the apoptosis.ResultsD‐allose significantly improved insulin secretion of islets. The stimulation index in group 2 was significantly higher than in group 1. Cure rate after transplantation in group 2 was higher than in group 1 especially in the first week. The malondialdehyde level in group 2 was significantly lower than in group 1. But the caspase 3 activities in both groups did not differ.ConclusionsD‐allose treatment of isolated islet culture prior to transplantation restored islet function and increased successful transplant rate. The results of this study suggested that D‐allose improved function of damaged islets through its anti‐oxidative activity.