Glucose deprivation and hypoxia increase the expression of the GLUT1 glucose transporter via a specific mRNA cis-acting regulatory element
Glucose deprivation and hypoxia increase the expression of the GLUT1 glucose transporter via a specific mRNA cis-acting regulatory element
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DOI：
10.1046/j.0022-3042.2001.00756.x
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发表时间：
2002-02-01
影响因子：
4.7
通讯作者：
Pardridge, WM
中科院分区：
文献类型：
--
作者：
Boado, RJ;Pardridge, WM
葡萄糖转运蛋白1（GLUT 1）基因的表达在缺氧和葡萄糖剥夺中上调。位于GLUT 1 3 '-非翻译区（CAE（2181-2190））的nt 2181-2190内的10个核苷酸（nt）顺式作用调节元件（CAE）增加GLUT 1-荧光素酶报告基因的表达并减少其mRNA衰减。本研究使用稳定的转染子研究GLUT 1 CAE（2181-2190）在葡萄糖剥夺和缺氧中的作用。葡萄糖和O-2剥夺导致携带CAE（2181-2190）的GLUT 1报告基因的表达显著增加，并且这种效应是累加的。葡萄糖剥夺和/或缺氧未诱导报告基因表达的显著变化，其中GLUT 1 CAE（2181-2190）被定点缺失。本文提供的数据表明，GLUT 1 CAE（2181-2190）参与葡萄糖剥夺和缺氧时GLUT 1基因表达的增加。
The expression of the glucose transporter type-1 (GLUT1) gene is up-regulated in hypoxia and glucose deprivation. A 10 nucleotide (nt) cis-acting regulatory element (CAE), which is located within nt 2181-2190 of the GLUT1 3'-untranslated region (CAE(2181-2190)), increases the expression of a GLUT1-luciferase reporter gene and decreases its mRNA decay. The present study investigated the role of the GLUT1 CAE(2181-2190) in glucose deprivation and hypoxia using stable transfectants. Glucose and O-2 deprivation produced a marked increase in the expression of the GLUT1 reporter gene carrying the CAE(2181-2190), and this effect was additive. Glucose deprivation and/or hypoxia induced no significant changes in the expression of the reporter gene wherein the GLUT1 CAE(2181-2190) was site-directed deleted. Data presented here suggest that the GLUT1 CAE(2181-2190) participates in the increase of GLUT1 gene expression in glucose deprivation and hypoxia.